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Title: Altered glucose kinetics in diabetic rats during Gram-negative infection

Abstract

The present study examined the purported exacerbating effect of sepsis on glucose metabolism in diabetes. Diabetes was induced in rats by an intravenous injection of 70 or 45 mg/kg streptozotocin. The higher dose produced severe diabetes, whereas the lower dose of streptozotocin produced a miler, latent diabetes. After a chronic diabetic state had developed for 4 wk, rats had catheters implanted and sepsis induced by intraperitoneal injections of live Escherichia coli. After 24 h of sepsis the blood glucose concentration was unchanged in nondiabetics and latent diabetics, but glucose decreased from 15 to 8 mM in the septic severe diabetic group. This decrease in blood glucose was not accompanied by alterations in the plasma insulin concentration. Glucose turnover, assessed by the constant intravenous infusion of (6-{sup 3}H)- and (U-{sup 14}C)glucose, was elevated in the severe diabetic group, compared with either latent diabetics or nondiabetics. Sepsis increased the rate of glucose disappearance in nondiabetic rats but had no effect in either group of diabetic animals. Sepsis also failed to alter the insulinogenic index, used to estimate the insulin secretory capacity, in diabetic rats. Thus the present study suggests that the imposition of nonlethal Gram-negative sepsis on severe diabetic animals does notmore » further impair glucose homeostasis and that the milder latent diabetes was not converted to a more severe diabetic state by the septic challenge.« less

Authors:
; ; ;  [1]
  1. Louisiana State Univ. Medical Center, New Orleans (USA)
Publication Date:
OSTI Identifier:
5060146
Resource Type:
Journal Article
Journal Name:
American Journal of Physiology; (USA)
Additional Journal Information:
Journal Volume: 253:2; Journal ID: ISSN 0002-9513
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; DIABETES MELLITUS; PATHOGENESIS; GLUCOSE; METABOLISM; LABELLED COMPOUNDS; CARBON 14 COMPOUNDS; ESCHERICHIA COLI; HOMEOSTASIS; RATS; SEPTICEMIA; TRITIUM COMPOUNDS; ALDEHYDES; ANIMALS; BACTERIA; CARBOHYDRATES; DISEASES; ENDOCRINE DISEASES; HEXOSES; HYDROGEN COMPOUNDS; MAMMALS; METABOLIC DISEASES; MICROORGANISMS; MONOSACCHARIDES; ORGANIC COMPOUNDS; RODENTS; SACCHARIDES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Lang, C H, Dobrescu, C, Bagby, G J, and Spitzer, J J. Altered glucose kinetics in diabetic rats during Gram-negative infection. United States: N. p., 1987. Web.
Lang, C H, Dobrescu, C, Bagby, G J, & Spitzer, J J. Altered glucose kinetics in diabetic rats during Gram-negative infection. United States.
Lang, C H, Dobrescu, C, Bagby, G J, and Spitzer, J J. 1987. "Altered glucose kinetics in diabetic rats during Gram-negative infection". United States.
@article{osti_5060146,
title = {Altered glucose kinetics in diabetic rats during Gram-negative infection},
author = {Lang, C H and Dobrescu, C and Bagby, G J and Spitzer, J J},
abstractNote = {The present study examined the purported exacerbating effect of sepsis on glucose metabolism in diabetes. Diabetes was induced in rats by an intravenous injection of 70 or 45 mg/kg streptozotocin. The higher dose produced severe diabetes, whereas the lower dose of streptozotocin produced a miler, latent diabetes. After a chronic diabetic state had developed for 4 wk, rats had catheters implanted and sepsis induced by intraperitoneal injections of live Escherichia coli. After 24 h of sepsis the blood glucose concentration was unchanged in nondiabetics and latent diabetics, but glucose decreased from 15 to 8 mM in the septic severe diabetic group. This decrease in blood glucose was not accompanied by alterations in the plasma insulin concentration. Glucose turnover, assessed by the constant intravenous infusion of (6-{sup 3}H)- and (U-{sup 14}C)glucose, was elevated in the severe diabetic group, compared with either latent diabetics or nondiabetics. Sepsis increased the rate of glucose disappearance in nondiabetic rats but had no effect in either group of diabetic animals. Sepsis also failed to alter the insulinogenic index, used to estimate the insulin secretory capacity, in diabetic rats. Thus the present study suggests that the imposition of nonlethal Gram-negative sepsis on severe diabetic animals does not further impair glucose homeostasis and that the milder latent diabetes was not converted to a more severe diabetic state by the septic challenge.},
doi = {},
url = {https://www.osti.gov/biblio/5060146}, journal = {American Journal of Physiology; (USA)},
issn = {0002-9513},
number = ,
volume = 253:2,
place = {United States},
year = {Sat Aug 01 00:00:00 EDT 1987},
month = {Sat Aug 01 00:00:00 EDT 1987}
}