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Title: Genetic studies of cell fusion induced by herpes simplex virus type 1

Abstract

Eight cell fusion-causing syn mutants were isolated from the KOS strain of herpes simplex virus type 1. Unlike the wild-type virus, the mutants produced plaques containing multinucleated cells, or syncytia. Fusion kinetics curves were established with a Coulter Counter assay for the mutants and wild-type virus in single infections of human embryonic lung (HEL) cells, for the mutants and wild-type virus in mixed infections (dominance test), and for pairs of mutants in mixed infection and proceeded with an exponential decrease in the number of small single cells. At some later time that was characteristic of the mutant, there was a significant reduction in the rate of fusion for all but possibly one of the mutants. Although the wild-type virus did not produce syncytial plaques, it did induce a small amount of fusion that stopped abruptly about 2 h after it started. These data are consistent with the hypothesis that both mutants and wild type induce an active fusion inducer and that the activity of this inducer is subsequently inhibited. The extent of fusion is apparently determined by the length of the interval during which the fusion inducer is active. That fusion is actively inhibited in wild-type infections is indicated bymore » the observation that syn mutant-infected cells fused more readily with uninfected cells than with wild type-infected cells.« less

Authors:
; ;
Publication Date:
Research Org.:
Pennsylvania State Univ., University Park
OSTI Identifier:
5059817
DOE Contract Number:  
EY-76-S-02-3419
Resource Type:
Journal Article
Journal Name:
J. Virol.; (United States)
Additional Journal Information:
Journal Volume: 35:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HERPES SIMPLEX; BIOLOGICAL EFFECTS; CELL MEMBRANES; EMBRYONIC CELLS; GENETICS; INFECTIVITY; MUTANTS; PHENOTYPE; RESPIRATORY TRACT CELLS; TIME DEPENDENCE; ANIMAL CELLS; BIOLOGY; CELL CONSTITUENTS; DISEASES; INFECTIOUS DISEASES; MEMBRANES; SKIN DISEASES; 550700* - Microbiology; 550300 - Cytology

Citation Formats

Read, G S, Person, S, and Keller, P M. Genetic studies of cell fusion induced by herpes simplex virus type 1. United States: N. p., 1980. Web.
Read, G S, Person, S, & Keller, P M. Genetic studies of cell fusion induced by herpes simplex virus type 1. United States.
Read, G S, Person, S, and Keller, P M. 1980. "Genetic studies of cell fusion induced by herpes simplex virus type 1". United States.
@article{osti_5059817,
title = {Genetic studies of cell fusion induced by herpes simplex virus type 1},
author = {Read, G S and Person, S and Keller, P M},
abstractNote = {Eight cell fusion-causing syn mutants were isolated from the KOS strain of herpes simplex virus type 1. Unlike the wild-type virus, the mutants produced plaques containing multinucleated cells, or syncytia. Fusion kinetics curves were established with a Coulter Counter assay for the mutants and wild-type virus in single infections of human embryonic lung (HEL) cells, for the mutants and wild-type virus in mixed infections (dominance test), and for pairs of mutants in mixed infection and proceeded with an exponential decrease in the number of small single cells. At some later time that was characteristic of the mutant, there was a significant reduction in the rate of fusion for all but possibly one of the mutants. Although the wild-type virus did not produce syncytial plaques, it did induce a small amount of fusion that stopped abruptly about 2 h after it started. These data are consistent with the hypothesis that both mutants and wild type induce an active fusion inducer and that the activity of this inducer is subsequently inhibited. The extent of fusion is apparently determined by the length of the interval during which the fusion inducer is active. That fusion is actively inhibited in wild-type infections is indicated by the observation that syn mutant-infected cells fused more readily with uninfected cells than with wild type-infected cells.},
doi = {},
url = {https://www.osti.gov/biblio/5059817}, journal = {J. Virol.; (United States)},
number = ,
volume = 35:1,
place = {United States},
year = {Tue Jul 01 00:00:00 EDT 1980},
month = {Tue Jul 01 00:00:00 EDT 1980}
}