Permeabilization of ultraviolet-irradiated chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus

Yarosh, D B; Setlow, R B

doi:10.1128/MCB.1.3.237

Title: Permeabilization of ultraviolet-irradiated chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus

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Abstract

Chinese hamster V-79 cells were made permeable by treatment with polyethylene glycol and then incubated with a Micrococcus luteus extract containing ultraviolet-specific endonuclease activity. This treatment introduced nicks in irradiated, but not in unirradiated, deoxyribonucleic acid. The nicks remained open for at least 3 h; there was no loss of endonuclease-sensitive sites, and no excision of dimers as measured by chromatography was detected. In addition, there was no increase in ultraviolet resistance in treated cells. This suggests that the absence of a significant amount of excision repair in rodent cells is due to the lack of both incision and excision capacity.

Authors:: Yarosh, D B; Setlow, R B

Publication Date:: Sun Mar 01 00:00:00 EST 1981

Research Org.:: Brookhaven National Lab. (BNL), Upton, NY (United States)

OSTI Identifier:: 5046684

Resource Type:: Journal Article

Journal Name:: Mol. Cell. Biol.; (United States)

Additional Journal Information:: Journal Volume: 1:3

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; BIOLOGICAL RADIATION EFFECTS; FIBROBLASTS; PERMEABILITY; NUCLEASES; RADIOSENSITIVITY EFFECTS; BIOLOGICAL REPAIR; CARBON 14 COMPOUNDS; DIMERS; EXTREME ULTRAVIOLET RADIATION; HAMSTERS; MICROCOCCUS LUTEUS; POLYETHYLENE GLYCOLS; RADIOINDUCTION; TRITIUM COMPOUNDS; ALCOHOLS; ANIMAL CELLS; ANIMALS; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; ENZYMES; ESTERASES; GLYCOLS; HYDROLASES; HYDROXY COMPOUNDS; LABELLED COMPOUNDS; MAMMALS; MICROCOCCUS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC POLYMERS; PHOSPHODIESTERASES; POLYMERS; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; RODENTS; SOMATIC CELLS; ULTRAVIOLET RADIATION; VERTEBRATES; 560121* - Radiation Effects on Cells- External Source- (-1987)

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                    Yarosh, D B, and Setlow, R B. Permeabilization of ultraviolet-irradiated chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus.  United States: N. p., 1981. 
        Web.  doi:10.1128/MCB.1.3.237.

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                    Yarosh, D B, & Setlow, R B. Permeabilization of ultraviolet-irradiated chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus.  United States.  https://doi.org/10.1128/MCB.1.3.237

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                    Yarosh, D B, and Setlow, R B. 1981.  
        "Permeabilization of ultraviolet-irradiated chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus".  United States.  https://doi.org/10.1128/MCB.1.3.237.

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@article{osti_5046684,

  title        = {Permeabilization of ultraviolet-irradiated chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus},

  author       = {Yarosh, D B and Setlow, R B},

  abstractNote = {Chinese hamster V-79 cells were made permeable by treatment with polyethylene glycol and then incubated with a Micrococcus luteus extract containing ultraviolet-specific endonuclease activity. This treatment introduced nicks in irradiated, but not in unirradiated, deoxyribonucleic acid. The nicks remained open for at least 3 h; there was no loss of endonuclease-sensitive sites, and no excision of dimers as measured by chromatography was detected. In addition, there was no increase in ultraviolet resistance in treated cells. This suggests that the absence of a significant amount of excision repair in rodent cells is due to the lack of both incision and excision capacity.},

  doi          = {10.1128/MCB.1.3.237},

  url          = {https://www.osti.gov/biblio/5046684},
  journal      = {Mol. Cell. Biol.; (United States)},
number       = ,

  volume       = 1:3,

  place        = {United States},

  year         = {Sun Mar 01 00:00:00 EST 1981},

  month        = {Sun Mar 01 00:00:00 EST 1981}

}

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