Direct effects of serotonin and ketanserin on the functional morphology of embryonic chick skin in vitro

Journal Article · Sun Oct 01 00:00:00 EDT 1989 · In Vitro Cellular and Developmental Biology; (USA)

DOI:https://doi.org/10.1007/BF02624005· OSTI ID:5045203

Beele, H; Thierens, H; de Ridder, L ^[1]

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1. State Univ. of Ghent (Belgium)

Different organotypical culture methods are used to test direct effects of serotonin and ketanserin, a S2, alpha 1, and H1 receptor antagonist in vascular tissue, on fibroblasts and epidermal cells of embryonic chick skin in vitro. From light microscopic and electron microscopic analyses, we learn that serotonin enhances keratinization and differentiation, whereas ketanserin reduces differentiation in comparison to the control cultures. Incorporation data of fragments cultured with (3H)thymidine show that ketanserin, within a dose range from 0.05 to 5 micrograms/ml, stimulates proliferation. Serotonin at a concentration of 10 micrograms/ml slightly slows down proliferation, whereas lower doses of 0.1 and 1 microgram/ml result in tritium activities that do not differ from control cultures.

• OSTI ID: 5045203
• Journal Information: In Vitro Cellular and Developmental Biology; (USA), Vol. 25:10; ISSN 0883-8364
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FIBROBLASTS
CELL PROLIFERATION
SEROTONIN
BIOLOGICAL EFFECTS
AUTORADIOGRAPHY
CHICKENS
DOSE-RESPONSE RELATIONSHIPS
ELECTRON MICROSCOPY
EMBRYOS
EPIDERMIS
IN VITRO
MORPHOLOGY
SCINTILLATION COUNTING
THYMIDINE
TRITIUM COMPOUNDS
AMINES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZINES
AZOLES
BIRDS
BODY
CONNECTIVE TISSUE CELLS
COUNTING TECHNIQUES
DRUGS
EPITHELIUM
FOWL
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
MICROSCOPY
NEUROREGULATORS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
PYRROLES
RADIOPROTECTIVE SUBSTANCES
RIBOSIDES
SKIN
SOMATIC CELLS
SYMPATHOMIMETICS
TISSUES
TRYPTAMINES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550301 - Cytology- Tracer Techniques