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Title: Tissue-specific and differentiation-specific expression of a human K14 keratin gene in transgenic mice

Abstract

A construct containing {approx}2,500 base pairs (bp) of 5{prime} upstream and {approx}700 bp of 3{prime} downstream sequence was used to drive the expression of an intronless human K14 gene in vitro and in vivo. To track the expression of the gene, a small sequence encoding the antigenic portion of neuropeptide substance P was inserted in frame 5{prime} to the TGA translation stop codon of the gene. Surprisingly, this gene was expressed promiscuously in a wide variety of cultured cells transiently transfected with the construct. In contrast, when introduced into the germ line of transgenic mice, the construct was expressed in a fashion analogous to the endogenous K14 gene--namely, in the basal layer of stratified squamous epithelia. The results suggest that some regulatory mechanism is overridden as a consequence of transient transfection but that sequences that can control proper K14 expression are present in the construct. The appropriate tissue-specific and differentiation-specific expression of K14{center dot}P in transgenic mice is an important first step in characterizing a promoter that could be employed to drive the foreign expression of drug-related genes in the epidermis of skin grafts.

Authors:
; ; ;  [1];  [2]
  1. Univ. of Chicago, IL (USA)
  2. Univ. of Illinois Medical School, Chicago (USA)
Publication Date:
OSTI Identifier:
5037076
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (USA)
Additional Journal Information:
Journal Volume: 86:5; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GENE REPRESSORS; DNA SEQUENCING; KERATIN; GENE REGULATION; BIOLOGICAL MARKERS; CELL DIFFERENTIATION; DNA HYBRIDIZATION; EPITHELIUM; GENES; MICE; PHOSPHORUS 32; ANIMAL TISSUES; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; HYBRIDIZATION; ISOTOPES; LIGHT NUCLEI; MAMMALS; NUCLEI; NUCLEOPROTEINS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; PROTEINS; RADIOISOTOPES; RODENTS; SCLEROPROTEINS; STRUCTURAL CHEMICAL ANALYSIS; TISSUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Vassar, R, Rosenberg, M, Tyner, A, Fuchs, E, and Ross, S. Tissue-specific and differentiation-specific expression of a human K14 keratin gene in transgenic mice. United States: N. p., 1989. Web. doi:10.1073/pnas.86.5.1563.
Vassar, R, Rosenberg, M, Tyner, A, Fuchs, E, & Ross, S. Tissue-specific and differentiation-specific expression of a human K14 keratin gene in transgenic mice. United States. https://doi.org/10.1073/pnas.86.5.1563
Vassar, R, Rosenberg, M, Tyner, A, Fuchs, E, and Ross, S. 1989. "Tissue-specific and differentiation-specific expression of a human K14 keratin gene in transgenic mice". United States. https://doi.org/10.1073/pnas.86.5.1563.
@article{osti_5037076,
title = {Tissue-specific and differentiation-specific expression of a human K14 keratin gene in transgenic mice},
author = {Vassar, R and Rosenberg, M and Tyner, A and Fuchs, E and Ross, S},
abstractNote = {A construct containing {approx}2,500 base pairs (bp) of 5{prime} upstream and {approx}700 bp of 3{prime} downstream sequence was used to drive the expression of an intronless human K14 gene in vitro and in vivo. To track the expression of the gene, a small sequence encoding the antigenic portion of neuropeptide substance P was inserted in frame 5{prime} to the TGA translation stop codon of the gene. Surprisingly, this gene was expressed promiscuously in a wide variety of cultured cells transiently transfected with the construct. In contrast, when introduced into the germ line of transgenic mice, the construct was expressed in a fashion analogous to the endogenous K14 gene--namely, in the basal layer of stratified squamous epithelia. The results suggest that some regulatory mechanism is overridden as a consequence of transient transfection but that sequences that can control proper K14 expression are present in the construct. The appropriate tissue-specific and differentiation-specific expression of K14{center dot}P in transgenic mice is an important first step in characterizing a promoter that could be employed to drive the foreign expression of drug-related genes in the epidermis of skin grafts.},
doi = {10.1073/pnas.86.5.1563},
url = {https://www.osti.gov/biblio/5037076}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (USA)},
issn = {0027-8424},
number = ,
volume = 86:5,
place = {United States},
year = {Wed Mar 01 00:00:00 EST 1989},
month = {Wed Mar 01 00:00:00 EST 1989}
}