Sequential expression and cooperative interaction of c-Ha-ras and c-erbB genes in in vivo chemical carcinogenesis

Journal Article · Wed Feb 01 00:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.4.1264· OSTI ID:5030972

Husain, Z; Fei, Y; Roy, S; Biswas, D K ^[1]; Solt, D B; Polverini, P J ^[2]

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1. Harvard School of Dental Medicine, Boston, MA (USA)
2. Northwestern Univ. Medical School, Chicago, IL (USA)

The level of expression of several cellular protooncogenes is examined at different stages of 7,12-dimethylbenzanthracene (DMBA)-induced tumor development in hamster buccal pouch epithelium (HBPE). Results presented demonstrate overexpression of c-Ha-ras gene at a very early stage of tumor development, and this elevated level of expression of the gene persists throughout the tumorigenesis process. The expression of the cellular protooncogene c-erbB, on the other hand, can be detected only after 8-10 weeks of DMBA treatment of the tissue and increases with the progression of the disease. The overexpression of c-erbB gene can be correlated with the stage of extensive proliferation and subsequent invasion of the HBPE cells into the underlying connective tissue. This sequential pattern of stage-specific expression of the two cellular protooncogenes can be observed in (i) treated tissues, (ii) stage-representative cultured cells, and (iii) NIH 3T3 transformants derived with DNA from HBPE cells. The sequential overexpression of c-Ha-ras and c-erbB genes in a stage-specific manner and their cooperative interaction in the DMBA-induced in vivo oral carcinogenesis have been demonstrated.

• OSTI ID: 5030972
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:4; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DIMETHYLBENZANTHRACENE
GENETIC EFFECTS
ONCOGENES
GENE REGULATION
CARCINOGENESIS
EPITHELIUM
HAMSTERS
MESSENGER-RNA
METHIONINE
MICE
PRECIPITATION
SULFUR 35
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
AROMATICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CONDENSED AROMATICS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
GENES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PATHOGENESIS
RADIOISOTOPES
RNA
RODENTS
SEPARATION PROCESSES
SULFUR ISOTOPES
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques