Endocytosis of simian virus 40 into the endoplasmic reticulum

Journal Article · Fri Dec 01 00:00:00 EST 1989 · Journal of Cell Biology; (USA)

DOI:https://doi.org/10.1083/jcb.109.6.2721· OSTI ID:5029938

Kartenbeck, J; Stukenbrok, H; Helenius, A ^[1]

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1. Yale School of Medicine, New Haven, CT (USA)

The endocytosis of SV-40 into CV-1 cells we studied using biochemical and ultrastructural techniques. The half-time of binding of ({sup 35}S)methionine-radiolabeled SV-40 to CV-1 cells was 25 min. Most of the incoming virus particles remained undegraded for several hours. Electron microscopy showed that some virus entered the endosomal/lysosomal pathway via coated vesicles, while the majority were endocytosed via small uncoated vesicles. After infection at high multiplicity, one third of total cell-associated virus was observed to enter the ER, starting 1-2 h after virus application. The viruses were present in large, tubular, smooth membrane networks generated as extentions of the ER. The results describe a novel and unique membrane transport pathway that allows endocytosed viral particles to be targeted from the plasma membrane to the ER.

• OSTI ID: 5029938
• Journal Information: Journal of Cell Biology; (USA), Vol. 109; ISSN 0021-9525
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
SIMIAN VIRUS
PHAGOCYTOSIS
AUTORADIOGRAPHY
BIOLOGICAL PATHWAYS
CELL MEMBRANES
ELECTRON MICROSCOPY
ENDOPLASMIC RETICULUM
MEMBRANE TRANSPORT
METHIONINE
SULFUR 35
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MEMBRANES
MICROORGANISMS
MICROSCOPY
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
PARASITES
RADIOISOTOPES
SULFUR ISOTOPES
VIRUSES
550301* - Cytology- Tracer Techniques