Microbial degradation of polychlorinated hydrocarbons. [Aspergillus, Serratia, Bacillus, Pseudomonas]
- Univ. of Georgia, Athens (USA)
Mutant strains of Asperqillus, Serratia, Pseudomonas, and Bacillus spp. were developed to resist polychlorinated biphenyls (PCBs) 1254. The mutant cells of Serratia and Pseudomonas spp. were not affected after 120 min exposure to 1 000 ppm PCB 1254/ml buffer, and grew well in glucose basal salt broth (GBSB) and 400 ppm PCB. Generation time of Pseudomonas in GBSB was 62 min in absence and 66 min in presence of 200 ppm PCB 1254. Maximal PCB uptake of 39.7% was noted for Pseudomonas cultures grown in basal salt broth (72 h, 37{degree}C) and most PCB was in lipid fraction of cell walls and membranes. Bioreactor experiments using 122 L of waste (800 mg PCB 1248/L) showed that Pseudomonas and Serratia spp. degraded most of the PCB in 90 - 130 days. PCB dechlorination occurred in Pseudomonas cell walls followed by metabolism to lower chlorinated molecules, CO{sub 2}, and H{sub 2}O in the cytoplasm via oxidative pathway. Activated charcoal (AC) was used to remove PCBs from waste, and repeated hexane extractions recovered the compounds from the AC, suggesting AC adsorption as a possible means for removing low levels of PCBs from industrial waste.
- OSTI ID:
- 5029767
- Journal Information:
- Proceedings of the Society for Experimental Biology and Medicine; (USA), Journal Name: Proceedings of the Society for Experimental Biology and Medicine; (USA); ISSN 0037-9727
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ASPERGILLUS
METABOLISM
BACILLUS
CHLORINATED AROMATIC HYDROCARBONS
BIODEGRADATION
PSEUDOMONAS
SERRATIA
INDUSTRIAL WASTES
MUTANTS
REMOVAL
UPTAKE
WASTE DISPOSAL
AROMATICS
BACTERIA
CHEMICAL REACTIONS
DECOMPOSITION
EUMYCOTA
FUNGI
HALOGENATED AROMATIC HYDROCARBONS
MANAGEMENT
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PLANTS
WASTE MANAGEMENT
WASTES
540220* - Environment
Terrestrial- Chemicals Monitoring & Transport- (1990-)