Reassessment of sup 14 CO sub 2 compartmentation and of ( sup 14 C)formate oxidation in rat liver

Journal Article · Sat Nov 25 00:00:00 EST 1989 · Journal of Biological Chemistry; (USA)

OSTI ID:5029690

Marsolais, C; Lafreniere, F; David, F; Dodgson, S J; Brunengraber, H ^[1]

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1. Univ. of Montreal, Quebec (Canada)

Our previous report had concluded that a fraction of ({sup 14}C)formate oxidation in liver occurs in the mitochondrion. This conclusion was based on the labeling patterns of urea and acetoacetate labeled via {sup 14}CO{sub 2} generated from ({sup 14}C)formate and other ({sup 14}C)substrates. We reassessed our interpretation in experiments conducted in (i) perifused mitochondria and (ii) isolated livers perfused with buffer containing ({sup 14}C)formate, ({sup 14}C)gluconolactone, {sup 14}CO{sub 2}, or NaH{sup 13}CO{sub 3}, in the absence and presence of acetazolamide, an inhibitor of carbonic anhydrase. Our data show that the cytosolic pools of bicarbonate and CO{sub 2} are not in isotopic equilibrium when {sup 14}CO{sub 2} is generated in the cytosol or is supplied as NaH{sup 14}CO3. We retract our earlier suggestion of a mitochondrial site of ({sup 14}C)formate oxidation.

• OSTI ID: 5029690
• Journal Information: Journal of Biological Chemistry; (USA), Vol. 264:33; ISSN 0021-9258
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FORMATES
METABOLISM
ACETOACETATES
CARBON 14 COMPOUNDS
CARBON DIOXIDE
ENZYME INHIBITORS
GLUCOSE
LIVER
MITOCHONDRIA
PERFUSED ORGANS
RATS
TRACER TECHNIQUES
UREA
ALDEHYDES
AMIDES
ANIMALS
BODY
CARBOHYDRATES
CARBON COMPOUNDS
CARBON OXIDES
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACID SALTS
CELL CONSTITUENTS
CHALCOGENIDES
DIGESTIVE SYSTEM
GLANDS
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
RODENTS
SACCHARIDES
VERTEBRATES
550501* - Metabolism- Tracer Techniques