Cellular heredity in haploid cultures of somatic cells, March 1968-April 1981. Final report

Technical Report · Mon Mar 01 00:00:00 EST 1982

OSTI ID:5028219

Freed, J J

An account is given of the development and application to cell-culture genetics of unique haploid cell lines from frog embryo developed in this laboratory. Since 1968, the main aim of this project has been to develop the haploid cell system for studies of mutagenesis in culture, particularly by ultraviolet radiation. In the course of this work we isolated chromosomally stable cell lines, derived and characterized a number of variants, and adapted cell hybridization and other methods to this material. Particular emphasis was placed on ultraviolet photobiology, including studies of cell survival, mutagenesis, and pathways of repair of uv-damaged DNA. Although at present less widely used for genetic experiments than mammalian cell lines, the frog cells offer the advantages of authentic haploidy and a favorable repertory of DNA repair pathways for study of uv mutagenesis.

• Research Organization: Institute for Cancer Research, Philadelphia, PA (USA). Fox Chase Cancer Center
• DOE Contract Number: AC02-76EV03110
• OSTI ID: 5028219
• Report Number(s): DOE/EV/03110-32; ON: DE82018433
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL REPAIR
BIOLOGICAL PATHWAYS
DNA
EMBRYONIC CELLS
CULTIVATION TECHNIQUES
ULTRAVIOLET RADIATION
MUTAGENESIS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
FROGS
GENETIC RADIATION EFFECTS
HAPLOIDY
MUTATIONS
RADIOINDUCTION
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLOIDY
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
VERTEBRATES
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