Endothelial cell damage after gamma-irradiation in vitro: impaired uptake of alpha-aminoisobutyric acid. [137Cs]
The effects of gamma-irradiation with a cesium-137 source were assessed on endothelial cells isolated from bovine pulmonary arteries and maintained in culture. The radiobiologic parameters that characterize the dose-response survival curve for these cells, expressed as mean +/- SD were found to be n . 1.6 +/- 0.2, Dq . 71 +/- 13 rads, and Do . 161 +/- 35 rads, indicating a relatively low capacity of these cells to accumulate or repair radiation damage. Increasing doses of radiation led to decreasing uptake by endothelial cells of (14C)2-aminoisobutyric acid (AIB), a Na+-dependent and nonmetabolizable amino acid. A dose of 500 rads, which caused marked inhibition of cell survival, resulted in a 45% decrease in AIB uptake at 6 h and a 69% decrease in uptake at 24 h after irradiation. No morphologic abnormality was noted in these cells by light microscopy at 24 h after this dose of radiation. Bovine pulmonary artery fibroblasts, on the other hand, showed no significant impairment in uptake of AIB 24 h after exposure to doses of radiation as high as 5,000 rads. The uptake by endothelial cells of (14C)1-aminocyclopentane-1-carboxylic acid, an amino acid transported by a Na+-independent process, was not influenced by 5,000 rads of radiation. Our studies show that endothelial cells are sensitive to radiation and that impaired Na+-dependent uptake of AIB represents an early event in radiation damage to the endothelial cell.
- Research Organization:
- Department of Therapeutic Radiology, Tufts-New England Medical Center, Boston, Massachusetts
- OSTI ID:
- 5027482
- Journal Information:
- Am. Rev. Respir. Dis.; (United States), Vol. 125:1
- Country of Publication:
- United States
- Language:
- English
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ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
CARBON 14 COMPOUNDS
UPTAKE
MONOCARBOXYLIC ACIDS
ARTERIES
BIOLOGICAL REPAIR
CESIUM 137
FIBROBLASTS
GAMMA RADIATION
IN VITRO
SURVIVAL CURVES
ALKALI METAL ISOTOPES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BLOOD VESSELS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CESIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
ISOTOPES
LABELLED COMPOUNDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
REPAIR
SOMATIC CELLS
YEARS LIVING RADIOISOTOPES
560121* - Radiation Effects on Cells- External Source- (-1987)