X-ray microanalysis of hamster tracheal epithelium

Journal Article · Thu Jun 01 00:00:00 EDT 1989 · Scanning Miscroscopy; (USA)

OSTI ID:5027422

Spencer, A J; Roomans, G M ^[1]

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1. Univ. of Uppsala (Sweden)

Studies of ion transport across respiratory epithelia are of great interest if we are to understand the pathophysiology of diseases such as cystic fibrosis in which ion transport is abnormal. Concentrations of elements were determined in various subcellular regions of normal or isoproterenol-treated hamster tracheal epithelium, using X-ray microanalysis of freeze-dried cryosections. Samples of trachea were taken from animals under anesthesia and either frozen in situ or dissected and plunge frozen. Concentrations of Mg, P, S, Cl, K and Ca were higher in cytoplasm and nuclei of control epithelial cells in dissected samples than in cryoneedle samples. Following treatment with isoproterenol, a large decrease in the concentration of Cl was observed. The results confirm that cyclic AMP-regulated chloride secretion is unaffected by anesthesia.

• OSTI ID: 5027422
• Journal Information: Scanning Miscroscopy; (USA), Vol. 3:2; ISSN 0891-7035
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
CALCIUM COMPOUNDS
MEMBRANE TRANSPORT
CHLORIDES
MANGANESE COMPOUNDS
PHOSPHORUS COMPOUNDS
POTASSIUM COMPOUNDS
TRACHEA
ELECTRON MICROPROBE ANALYSIS
ANESTHESIA
CELL MEMBRANES
CELL NUCLEI
CYTOPLASM
EPITHELIUM
HAMSTERS
ALKALI METAL COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
ANIMAL TISSUES
ANIMALS
BODY
CELL CONSTITUENTS
CHEMICAL ANALYSIS
CHLORINE COMPOUNDS
HALIDES
HALOGEN COMPOUNDS
MAMMALS
MEMBRANES
MICROANALYSIS
RESPIRATORY SYSTEM
RODENTS
TISSUES
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
550602* - Medicine- External Radiation in Diagnostics- (1980-)