Synthesis of diacylglycerol de novo is responsible for permanent activation and down-regulation of protein kinase C in transformed cells
- Univ. of Firenze (Italy)
We measured the synthesis of diacylglycerol de novo in normal NIH/3T3 fibroblasts and in cells transformed by ras, src, sis and abl oncogenes. Analysis of the incorporation of glucose-derived {sup 14}C into diacylglycerol indicated that neosynthesis of diacylglycerol was constitutively active in the transformed cell lines. Elevated levels of diacylglycerol and persistent activation/down-regulation of protein kinase C reduced the binding of phorbol dibutyrate to transformed cells. This phenomenon could be reversed by blocking the glycolytic pathway, thus indicating that neosynthesized diacylglycerol was responsible for persistent activation and down-regulation of protein kinase C. In transformed cells, protein kinase C activity could not be stimulated by the addition of diolein; however, inhibition of glycolysis restored the ability of transformed cells to respond to diolein. Taken together these data indicate that constitutive synthesis of diacylglycerol de novo is responsible for activation and down-regulation of protein kinase C in transformed cells, and it may play a role in altered mitogenic signalling.
- OSTI ID:
- 5026814
- Journal Information:
- Biochemical and Biophysical Research Communications; (USA), Vol. 164:2; ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Intracellular activation of protein kinase C and regulation of the surface transferrin receptor by diacylglycerol is a spontaneously reversible process that is associated with rapid formation of phosphatidic acid
Bryostatins: potent, new activators of protein kinase C
Related Subjects
GLUCOSE
METABOLISM
GLYCEROL
BIOSYNTHESIS
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
FIBROBLASTS
MICE
ONCOGENES
ONCOGENIC TRANSFORMATIONS
RECEPTORS
TRACER TECHNIQUES
ALCOHOLS
ALDEHYDES
ANIMAL CELLS
ANIMALS
CARBOHYDRATES
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
ENZYMES
GENES
HEXOSES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
RODENTS
SACCHARIDES
SOMATIC CELLS
SYNTHESIS
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques