Development and characterization of a homologous radioimmunoassay for deer mouse (Peromyscus maniculatus bairdii) prolactin

Journal Article · Mon Dec 05 00:00:00 EST 1983 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(83)90264-3· OSTI ID:5022072

Marr, G A; Colosi, P; Desjardins, C; Talamantes, F

A highly specific and sensitive homologous radioimmunoassay has been developed for the secreted form of prolactin from the deer mouse Peromyscus maniculatus bairdii. Peromyscus serum and pituitary homogenates displayed paralled dilution response curves, and no cross reaction was seen with either mouse prolactin, mouse growth hormone or rat prolactin. The assay was sensitive to 25 picograms per tube and the intra- and inter-assay coefficients of variation were 5 and 3.6%, respectively. In addition, the authors have demonstrated that Peromyscus prolactin does not show parallel displacement in a homologous radioimmunoassay utilized for measuring prolactin in the common laboratory mouse.

• Research Organization: Univ. of California, Santa Cruz
• OSTI ID: 5022072
• Journal Information: Life Sci.; (United States), Vol. 33:23
• Country of Publication: United States
• Language: English

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550601* - Medicine- Unsealed Radionuclides in Diagnostics