Do cholinephosphotransferase and phosphatidylethanolamine methyltransferase synthesize different species of phosphatidylcholine
Two pathways exist for phosphatidylcholine (PC) synthesis in castor bean endosperm. The major pathway utilizes the reaction; (CDPcholine + diacylglycerol ..-->.. PC + CMP) while the other is through (PE + 3 S-Adenosylmethioninie ..-->.. PC + 3 homocysteine). The reason for two pathways is not clear. In an effort to determine if they produce two different products, radioactive precursors (SAM and CDPcholine) were administered to isolated endoplasmic reticulum from the castor bean endosperm. The products were extracted, chromatographed on TLC, and the PC classes separated by argentation chromatography. The radioactivity was determined by a RTLC Scanner. By these methods, it has been determined that there are differences between the PC products of the methyltransferase and the cholinephosphotransferase.
- Research Organization:
- Louisiana State Univ., Baton Rouge
- OSTI ID:
- 5021334
- Journal Information:
- Plant Physiol., Suppl.; (United States), Vol. 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986; Other Information: C45.00*
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ENDOSPERM
PHOSPHOLIPIDS
BIOSYNTHESIS
BIOLOGICAL PATHWAYS
ENZYMES
LABELLED POOL TECHNIQUES
LABELLING
PHASEOLUS
BACTERIA
BODY
ESTERS
ISOTOPE APPLICATIONS
LEGUMINOSAE
LIPIDS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PLANT TISSUES
PLANTS
RHIZOBIUM
SYNTHESIS
TISSUES
TRACER TECHNIQUES
550200* - Biochemistry