Guide to establishing water potential of aqueous two-phase solutions (polyethylene glycol plus dextran) by amendment with mannitol

Journal Article · Sat Jan 01 00:00:00 EST 1983 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.72.1.60· OSTI ID:5020936

Michel, B E; Wiggins, O K; Outlaw, Jr, W H

A practical guide to calculating the mannitol (MAN) amendment required to achieve the desired water potential (PSI) of polyethylene glycol/dextran (PEG/DEX) aqueous two-phase systems for protoplast purification is presented. The empirically generated equation PSI = 305(PEG')/sup 2/(MAN) + 0.74(PEG')(MAN)T - 103(PEG')(MAN) + 5.6(PEG')/sup 2/T - 623(PEG')/sup 2/ - 0.25(PEG')T + 12.7(PEG') - 0.078(MAN)T - 22.75(MAN) accurately predicts experimental PSI (in bars). The equation is applicable for these ranges: (PEG') from 0.047 to 0.13 gram per gram H/sub 2/O; (MAN) from 0 to 0.7 molal; T from 4.5 to 40/sup 0/C. The PSI for solutions of MAN, of PEG, and of DEX were also determined. Equations to fit data for each were generated. Analyses indicated a significant synergistic effect on PSI when MAN is added to PEG/DEX and, at certain concentrations, between PEG and DEX.

• Research Organization: Univ. of Georgia, Athens, GA (United States)
• OSTI ID: 5020936
• Journal Information: Plant Physiol.; (United States), Vol. 72
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DEXTRAN
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PLANT CELLS
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POLYETHYLENE GLYCOLS
AQUEOUS SOLUTIONS
ALCOHOLS
BLOOD SUBSTITUTES
CARBOHYDRATES
CHEMISTRY
DISPERSIONS
DRUGS
GLYCOLS
HEMATOLOGIC AGENTS
HYDROXY COMPOUNDS
MIXTURES
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