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Title: Endocrinological control and cellular localization of rat testicular angiotensin-converting enzyme (EC 3. 4. 15. 1)

Abstract

Hypophysectomy of prepubescent (3-week-old) rats prevented the pubertal development of testicular, but not pulmonary, angiotensin-converting enzyme (EC 3.4.15.1). Additionally, hypophysectomy resulted in a loss of testicular converting enzyme activity in 10-week-old rats that had achieved puberty and had developed enzyme activity. Hormone regimens consisting of FSH/LH (7.5 U/rat X day), hCG (10 U/rat X day), or testosterone (1 mg/rat X day) were employed to ascertain their ability to maintain activity in hypophysectomized rats. All three of the above hormone regimens, if initiated on the first day after hypophysectomy of 10-week-old rats, were capable of maintaining testicular converting enzyme activity. Centrifugal elutriation of dispersed testicular cells indicated that the majority of enzyme activity in mature rats was associated with the germinal cells, a result consistent with the data accumulated from the hormonal studies. Lastly, (/sup 3/H)captopril bound specifically to cellular fractions enriched in germinal cells. The above studies suggest that the pituitary gland is required for the development and maintenance of testicular angiotensin-converting enzyme in the rat by stimulating steroidogenesis in the testes. Furthermore, the sensitivity of converting enzyme activity to androgen coupled with the centrifugal elutriation and (/sup 3/H) captopril binding studies strongly support the notion that testicular converting enzymemore » is associated with germinal cells.« less

Authors:
; ; ; ; ;
Publication Date:
OSTI Identifier:
5020560
Resource Type:
Journal Article
Journal Name:
Endocrinology; (United States)
Additional Journal Information:
Journal Volume: 6
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PEPTIDE HYDROLASES; ENZYME ACTIVITY; TESTES; ANGIOTENSIN; CENTRIFUGATION; FSH; HCG; HYPOPHYSECTOMY; LH; RATS; SPERMATOZOA; TESTOSTERONE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANDROGENS; ANDROSTANES; ANIMALS; BODY; CARDIOVASCULAR AGENTS; DRUGS; ENZYMES; GAMETES; GERM CELLS; GLOBULINS; GONADOTROPINS; GONADS; HORMONES; HYDROLASES; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; KETONES; LABELLED COMPOUNDS; MALE GENITALS; MAMMALS; MEDICINE; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PITUITARY HORMONES; PROTEINS; RODENTS; SEPARATION PROCESSES; STEROID HORMONES; STEROIDS; SURGERY; VASOCONSTRICTORS; VERTEBRATES; 550501* - Metabolism- Tracer Techniques; 551001 - Physiological Systems- Tracer Techniques

Citation Formats

Velletri, P A, Aquilano, D R, Bruckwick, E, Tsai-Morris, C H, Dufau, M L, and Lovenberg, W. Endocrinological control and cellular localization of rat testicular angiotensin-converting enzyme (EC 3. 4. 15. 1). United States: N. p., 1985. Web.
Velletri, P A, Aquilano, D R, Bruckwick, E, Tsai-Morris, C H, Dufau, M L, & Lovenberg, W. Endocrinological control and cellular localization of rat testicular angiotensin-converting enzyme (EC 3. 4. 15. 1). United States.
Velletri, P A, Aquilano, D R, Bruckwick, E, Tsai-Morris, C H, Dufau, M L, and Lovenberg, W. 1985. "Endocrinological control and cellular localization of rat testicular angiotensin-converting enzyme (EC 3. 4. 15. 1)". United States.
@article{osti_5020560,
title = {Endocrinological control and cellular localization of rat testicular angiotensin-converting enzyme (EC 3. 4. 15. 1)},
author = {Velletri, P A and Aquilano, D R and Bruckwick, E and Tsai-Morris, C H and Dufau, M L and Lovenberg, W},
abstractNote = {Hypophysectomy of prepubescent (3-week-old) rats prevented the pubertal development of testicular, but not pulmonary, angiotensin-converting enzyme (EC 3.4.15.1). Additionally, hypophysectomy resulted in a loss of testicular converting enzyme activity in 10-week-old rats that had achieved puberty and had developed enzyme activity. Hormone regimens consisting of FSH/LH (7.5 U/rat X day), hCG (10 U/rat X day), or testosterone (1 mg/rat X day) were employed to ascertain their ability to maintain activity in hypophysectomized rats. All three of the above hormone regimens, if initiated on the first day after hypophysectomy of 10-week-old rats, were capable of maintaining testicular converting enzyme activity. Centrifugal elutriation of dispersed testicular cells indicated that the majority of enzyme activity in mature rats was associated with the germinal cells, a result consistent with the data accumulated from the hormonal studies. Lastly, (/sup 3/H)captopril bound specifically to cellular fractions enriched in germinal cells. The above studies suggest that the pituitary gland is required for the development and maintenance of testicular angiotensin-converting enzyme in the rat by stimulating steroidogenesis in the testes. Furthermore, the sensitivity of converting enzyme activity to androgen coupled with the centrifugal elutriation and (/sup 3/H) captopril binding studies strongly support the notion that testicular converting enzyme is associated with germinal cells.},
doi = {},
url = {https://www.osti.gov/biblio/5020560}, journal = {Endocrinology; (United States)},
number = ,
volume = 6,
place = {United States},
year = {Sat Jun 01 00:00:00 EDT 1985},
month = {Sat Jun 01 00:00:00 EDT 1985}
}