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Title: Microclones derived from the mouse chromosome 7 D-E bands map within the proximal region of the c14CoS deletion in albino mutant mice

Abstract

A group of radiation-induced perinatal-lethal deletions that include the albino (c) locus on mouse chromosome 7 causes failure of expression of various hepatocyte-specific genes when homozygous. The transcription of such genes could be controlled in trans by a regulatory gene(s) located within the proximal region of the C14CoS deletion. To identify this potential regulatory gene, a microclone library was established from microdissected D and E bands of chromosome 7. Three nonoverlapping microclones (E305, E336B, and E453B) hybridizing with wildtype but not with C14CoS/C14CoS DNA were isolated. E336B represents a single-copy DNA fragment, whereas E305 and E453B hybridized with 3 and 10 EcoRI DNA restriction fragments, respectively. All fragments map exclusively within the deletion. The microclones hybridized to DNA of viable C6H/C14CoS deletion heterozygotes but not to DNA of homozygotes for the lethal mutation c10R75M, which belongs to the same complementation group as c14CoS. DNA of viable homozygous mutant C62DSD, which carries a deletion breakpoint proximal to that of c6H, hybridized only with E453B. This microclone identified 6 EcoRI restriction fragments in C62DSD/C62DSD DNA. The results demonstrate that of the isolated microclones, E453B identifies a locus (D7RT453B) that maps closest to the hsdr-1 (hepatocyte-specific developmental regulation) locus, which maps between themore » proximal breakpoints of deletions c10R75M and c62DSD.« less

Authors:
; ; ; ; ; ;  [1]
  1. Department of Cell Biology, Fraunhofer Institute of Toxicology and Aerosol Research, Hannover (West Germany)
Publication Date:
OSTI Identifier:
5018197
Resource Type:
Journal Article
Journal Name:
Genomics; (United States)
Additional Journal Information:
Journal Volume: 10:3; Journal ID: ISSN 0888-7543
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CLONE CELLS; GENETIC MAPPING; GENE MUTATIONS; RADIOINDUCTION; LIVER CELLS; GENE REGULATION; CHROMOSOMES; DNA HYBRIDIZATION; GENES; GLUCOCORTICOIDS; LETHAL MUTATIONS; MICE; MUTANTS; ADRENAL HORMONES; ANIMAL CELLS; ANIMALS; CELL CULTURES; CORTICOSTEROIDS; HORMONES; HYBRIDIZATION; HYDROXY COMPOUNDS; KETONES; MAMMALS; MAPPING; MUTATIONS; ORGANIC COMPOUNDS; PREGNANES; RODENTS; SOMATIC CELLS; STEROID HORMONES; STEROIDS; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals

Citation Formats

Toenjes, R R.W., Weith, A, Rinchik, E M, Winking, H, Carnwath, J W, Kaliner, B, and Paul, D. Microclones derived from the mouse chromosome 7 D-E bands map within the proximal region of the c14CoS deletion in albino mutant mice. United States: N. p., 1991. Web. doi:10.1016/0888-7543(91)90453-L.
Toenjes, R R.W., Weith, A, Rinchik, E M, Winking, H, Carnwath, J W, Kaliner, B, & Paul, D. Microclones derived from the mouse chromosome 7 D-E bands map within the proximal region of the c14CoS deletion in albino mutant mice. United States. https://doi.org/10.1016/0888-7543(91)90453-L
Toenjes, R R.W., Weith, A, Rinchik, E M, Winking, H, Carnwath, J W, Kaliner, B, and Paul, D. 1991. "Microclones derived from the mouse chromosome 7 D-E bands map within the proximal region of the c14CoS deletion in albino mutant mice". United States. https://doi.org/10.1016/0888-7543(91)90453-L.
@article{osti_5018197,
title = {Microclones derived from the mouse chromosome 7 D-E bands map within the proximal region of the c14CoS deletion in albino mutant mice},
author = {Toenjes, R R.W. and Weith, A and Rinchik, E M and Winking, H and Carnwath, J W and Kaliner, B and Paul, D},
abstractNote = {A group of radiation-induced perinatal-lethal deletions that include the albino (c) locus on mouse chromosome 7 causes failure of expression of various hepatocyte-specific genes when homozygous. The transcription of such genes could be controlled in trans by a regulatory gene(s) located within the proximal region of the C14CoS deletion. To identify this potential regulatory gene, a microclone library was established from microdissected D and E bands of chromosome 7. Three nonoverlapping microclones (E305, E336B, and E453B) hybridizing with wildtype but not with C14CoS/C14CoS DNA were isolated. E336B represents a single-copy DNA fragment, whereas E305 and E453B hybridized with 3 and 10 EcoRI DNA restriction fragments, respectively. All fragments map exclusively within the deletion. The microclones hybridized to DNA of viable C6H/C14CoS deletion heterozygotes but not to DNA of homozygotes for the lethal mutation c10R75M, which belongs to the same complementation group as c14CoS. DNA of viable homozygous mutant C62DSD, which carries a deletion breakpoint proximal to that of c6H, hybridized only with E453B. This microclone identified 6 EcoRI restriction fragments in C62DSD/C62DSD DNA. The results demonstrate that of the isolated microclones, E453B identifies a locus (D7RT453B) that maps closest to the hsdr-1 (hepatocyte-specific developmental regulation) locus, which maps between the proximal breakpoints of deletions c10R75M and c62DSD.},
doi = {10.1016/0888-7543(91)90453-L},
url = {https://www.osti.gov/biblio/5018197}, journal = {Genomics; (United States)},
issn = {0888-7543},
number = ,
volume = 10:3,
place = {United States},
year = {Mon Jul 01 00:00:00 EDT 1991},
month = {Mon Jul 01 00:00:00 EDT 1991}
}