Radiation-induced neoplastic transformation of C3H10T1/2 cells is suppressed by ascorbic acid
- National Institute of Radiological Sciences, Chiba (Japan)
X-ray induced transformation of C3H10T1/2 cells was suppressed in a concentration-dependent manner by administration of ascorbic acid after irradiation (0.1-20 micrograms/ml for the first week) in the culture medium. The dose-response curve was shifted about 60% downward and was slightly steeper in the presence of ascorbic acid (5 micrograms/ml for the first week) than in its absence. The 1-week treatment procedure revealed that cells initiated by radiation remained susceptible to ascorbic acid until the time of morphological phenotype expression. The neoplastically transformed phenotype expressed after incubation for 8 weeks could no longer be suppressed by ascorbic acid even after culture transfer. Similarly, the neoplastically transformed phenotype suppressed for 8 weeks by ascorbic acid treatment was not subsequently expressed in the absence of ascorbic acid. On the basis of the oxygen-detoxifying nature of ascorbic acid, we postulated that expression of the neoplastically transformed phenotype is promoted by reactive oxygen species and peroxy radicals generated in cells during the whole assay period. The data may be useful as a guide for chemopreventive efforts against radiation carcinogenesis.
- OSTI ID:
- 5018064
- Journal Information:
- Radiation Research; (USA), Vol. 120:3; ISSN 0033-7587
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ASCORBIC ACID
RADIOSENSITIVITY EFFECTS
ONCOGENIC TRANSFORMATIONS
RADIOINDUCTION
CARCINOGENESIS
DOSE-RESPONSE RELATIONSHIPS
MICE
PHENOTYPE
RADICALS
X RADIATION
ANIMALS
CELL TRANSFORMATIONS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MAMMALS
PATHOGENESIS
RADIATIONS
RODENTS
VERTEBRATES
VITAMINS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture