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Title: Cardiac fibroblasts are predisposed to convert into myocyte phenotype: Specific effect of transforming growth factor. beta

Abstract

Cardiac fibroblasts are mainly responsible for the synthesis of major extracellular matrix proteins in the heart, including fibrillar collagen types I and III and fibronectin. In this report we show that these cells, when stimulated by transforming growth factor {beta}{sub 1} (TGF-{beta}{sub 1}), acquire certain myocyte-specific properties. Cultured cardiac fibroblasts from adult rabbit heart were treated with TGF-{beta}{sub 1}, (10-15 ng/ml) for different periods of time. Northern hybridization analysis of total RNA showed that cells treated with TGF-{beta}{sub 1} became stained with a monoclonal antibody to muscle-specific actin. After treatment of quiescent cells with TGF-{beta}{sub 1}, cell proliferation (as measured by ({sup 3}H)thymidine incorporation) was moderately increased. Cultured cardiac fibroblasts at the subconfluent stage, when exposed to TGF-{beta}{sub 1} in the presence of 10% fetal bovine serum, gave rise to a second generation of slowly growing cells that expressed muscle-specific actin filaments. The findings demonstrate that cardiac fibroblasts can be made to differentiate into cells that display many characteristics of cardiac myocytes. TGF-{beta}{sub 1} seems to be a specific inducer of such conversion.

Authors:
; ; ;  [1]
  1. Univ. of Chicago, IL (United States)
Publication Date:
OSTI Identifier:
5016266
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (United States)
Additional Journal Information:
Journal Volume: 88:3; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; FIBROBLASTS; MORPHOLOGY; GROWTH FACTORS; BIOLOGICAL EFFECTS; ACTIN; CELL DIFFERENTIATION; DNA HYBRIDIZATION; ENDOTHELIUM; HEART; MESSENGER-RNA; PHENOTYPE; PHORBOL ESTERS; PHOSPHORUS 32; RABBITS; STEM CELLS; THYMIDINE; TRITIUM COMPOUNDS; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; AZINES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CARCINOGENS; CARDIOVASCULAR SYSTEM; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; ESTERS; HETEROCYCLIC COMPOUNDS; HYBRIDIZATION; HYDROGEN COMPOUNDS; ISOTOPES; LIGHT NUCLEI; MAMMALS; MITOGENS; NUCLEI; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; PROTEINS; PYRIMIDINES; RADIOISOTOPES; RIBOSIDES; RNA; SOMATIC CELLS; TISSUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Eghbali, M, Tomek, R, Woods, C, and Bhambi, B. Cardiac fibroblasts are predisposed to convert into myocyte phenotype: Specific effect of transforming growth factor. beta. United States: N. p., 1991. Web. doi:10.1073/pnas.88.3.795.
Eghbali, M, Tomek, R, Woods, C, & Bhambi, B. Cardiac fibroblasts are predisposed to convert into myocyte phenotype: Specific effect of transforming growth factor. beta. United States. https://doi.org/10.1073/pnas.88.3.795
Eghbali, M, Tomek, R, Woods, C, and Bhambi, B. 1991. "Cardiac fibroblasts are predisposed to convert into myocyte phenotype: Specific effect of transforming growth factor. beta". United States. https://doi.org/10.1073/pnas.88.3.795.
@article{osti_5016266,
title = {Cardiac fibroblasts are predisposed to convert into myocyte phenotype: Specific effect of transforming growth factor. beta},
author = {Eghbali, M and Tomek, R and Woods, C and Bhambi, B},
abstractNote = {Cardiac fibroblasts are mainly responsible for the synthesis of major extracellular matrix proteins in the heart, including fibrillar collagen types I and III and fibronectin. In this report we show that these cells, when stimulated by transforming growth factor {beta}{sub 1} (TGF-{beta}{sub 1}), acquire certain myocyte-specific properties. Cultured cardiac fibroblasts from adult rabbit heart were treated with TGF-{beta}{sub 1}, (10-15 ng/ml) for different periods of time. Northern hybridization analysis of total RNA showed that cells treated with TGF-{beta}{sub 1} became stained with a monoclonal antibody to muscle-specific actin. After treatment of quiescent cells with TGF-{beta}{sub 1}, cell proliferation (as measured by ({sup 3}H)thymidine incorporation) was moderately increased. Cultured cardiac fibroblasts at the subconfluent stage, when exposed to TGF-{beta}{sub 1} in the presence of 10% fetal bovine serum, gave rise to a second generation of slowly growing cells that expressed muscle-specific actin filaments. The findings demonstrate that cardiac fibroblasts can be made to differentiate into cells that display many characteristics of cardiac myocytes. TGF-{beta}{sub 1} seems to be a specific inducer of such conversion.},
doi = {10.1073/pnas.88.3.795},
url = {https://www.osti.gov/biblio/5016266}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (United States)},
issn = {0027-8424},
number = ,
volume = 88:3,
place = {United States},
year = {Fri Feb 01 00:00:00 EST 1991},
month = {Fri Feb 01 00:00:00 EST 1991}
}