Southern analysis of genomic alterations in gamma-ray-induced aprt- hamster cell mutants
The role of genomic alterations in mutagenesis induced by ionizing radiation has been the subject of considerable speculation. By Southern blotting analysis we show here that 9 of 55 (approximately 1/6) gamma-ray-induced mutants at the adenine phosphoribosyl transferase (aprt) locus of Chinese hamster ovary (CHO) cells have a detectable genomic rearrangement. These fall into two classes: intragenic deletions and chromosomal rearrangements. In contrast, no major genomic alterations were detected among 67 spontaneous mutants, although two restriction site loss events were observed. Three gamma-ray-induced mutants were found to be intragenic deletions; all may have identical break-points. The remaining six gamma-ray-induced mutants demonstrating a genomic alteration appear to be the result of chromosomal rearrangements, possibly translocation or inversion events. None of the remaining gamma-ray-induced mutants showed any observable alteration in blotting pattern indicating a substantial role for point mutation in gamma-ray-induced mutagenesis at the aprt locus.
- OSTI ID:
- 5015717
- Journal Information:
- Genetics; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHO CELLS
GENETIC RADIATION EFFECTS
CHROMOSOMAL ABERRATIONS
DNA
GAMMA RADIATION
MOLECULAR WEIGHT
MUTAGENESIS
MUTANTS
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
IONIZING RADIATIONS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
560121* - Radiation Effects on Cells- External Source- (-1987)