Genomically imposed and somatically modified human thymocyte V sub. beta. gene repertoires

Journal Article · Mon Apr 01 00:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.7.2908· OSTI ID:5015319

Baccala, R; Kono, D H; Balderas, R S; Theofilopoulos, A N ^[1]; Walker, S ^[2]

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1. Research Inst. of Scripps Clinic, La Jolla, CA (United States)
2. Univ. of Southern California School of Medicine, Los Angeles (United States)

The effect of thymic selection on the expressed human T-cell antigen receptor {beta}-chain variable region (V{sub {beta}}) gene repertoire was examined by using a multiprobe RNase protection assay. The relative abundance of transcripts for 22 V{sub {beta}} genes (encompassing 17 of the 20 human V{sub {beta}} gene subfamilies) within a thymus, and among 17 thymuses, was variable. On the basis of the presence of corresponding mRNAs, no genomic deletions were detected, but several coding region polymorphisms were identified. Analysis of mature T-cell subsets revealed the absence of complete superantigen-mediated V{sub {beta}} deletions, suggesting that this phenomenon, in contrast to mouse, is uncommon or absent in humans. However, several V{sub {beta}} genes were over- or underexpressed in one or both mature single-positive (CD4{sup +}8{sup {minus}} or CD8{sup +}4{sup {minus}}) thymocyte subsets compared to syngeneic total, mostly immature thymocytes. Whether these changes are induced by relatively weak superantigens or conventional antigens and whether the downshifts are caused by negative selection or lack of positive selection remains to be determined.

• OSTI ID: 5015319
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:7; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
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