Effect of luminal or circulating nitrite on colonic ion movement in the rat

Journal Article · Sat Aug 01 00:00:00 EDT 1987 · American Journal of Physiology; (USA)

OSTI ID:5014819

Radcliffe, B C; Nance, S H; Deakin, E J; Roediger, W E.W. ^[1]

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1. Univ. of Adelaide, Woodville (Australia)

The disposition of intravenously or luminally administered nitrite across the colonic mucosa and its effect on ion movement into or from the colon was assessed in anesthetized Porton rats using the isolated colon instilled either with sodium chloride or sodium chloride with sodium butyrate. Ionic changes in the colon after intravenous injection of 10 {mu}mol NaNO{sub 2} were compared with those occurring after injection of 10 {mu}mol NaCl. After intravenous administration of nitrite, both nitrite and nitrate appeared in the colonic instillate in a ratio of 1:1. Nitrite increased chloride absorption (110%) and bicarbonate production (20%) when 40 mM butyrate was included in the instillate. Net sodium absorption, measured in the whole colon, was unchanged. Intravenous nitrite had no effect on ionic movement in the absence of butyrate. When NaNO{sub 2} was included luminally with the sodium chloride-butyrate instillate, bicarbonate production rate increased, but sodium and chloride absorption were unaffected. Nitrite concentration in the instillate decreased during the 40-min experimental period at a rate of 0.275 nmol{center dot}min{sup {minus}1}{center dot}cm{sup {minus}2} and nitrate appeared at a rate of 0.037 nmol{center dot}min{sup {minus}1}{center dot}cm{sup {minus}2}. The authors conclude that nitrite stimulates bicarbonate production in the colon, probably by stimulating the oxidation by butyrate, the main source of CO{sub 2} generation by the colonic mucosa.

• OSTI ID: 5014819
• Journal Information: American Journal of Physiology; (USA), Vol. 253:2; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ACID CARBONATES
BIOSYNTHESIS
CHROMIUM 51
TRACER TECHNIQUES
IONS
INTESTINAL ABSORPTION
NITRITES
BIOLOGICAL EFFECTS
EDTA
LARGE INTESTINE
MUCOUS MEMBRANES
SODIUM CHLORIDES
VOLUME
ABSORPTION
ALKALI METAL COMPOUNDS
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CHARGED PARTICLES
CHELATING AGENTS
CHLORIDES
CHLORINE COMPOUNDS
CHROMIUM ISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
HALIDES
HALOGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTESTINES
ISOTOPE APPLICATIONS
ISOTOPES
MEMBRANES
NITROGEN COMPOUNDS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
RADIOISOTOPES
SODIUM COMPOUNDS
SYNTHESIS
UPTAKE
560300* - Chemicals Metabolism & Toxicology
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