Structure of the O-specific, sialic acid containing polysaccharide chain and its linkage to the core region in lipopolysaccharide from Hafnia alvei strain 2 as elucidated by chemical methods, gas-liquid chromatography/mass spectrometry, and sup 1 H NMR spectroscopy

Journal Article · Tue May 21 00:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00234a027· OSTI ID:5014341

Gamian, A; Romanowska, E ^[1]; Dabrowski, U; Dabrowski, J ^[2]

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1. Ludwik Hirszfeld Inst., Wroclaw (Poland)
2. Max-Planck-Inst. fuer Medizinische Forschung (West Germany)

Mild acid hydrolysis of Hafnia alvei strain 2 lipopolysaccharide released no O-specific polysaccharide but instead gave a monomeric octasaccharide repeating unit with N-acetylneuraminic acid as the reducing terminus. In addition, a dimer of the octasaccharide repeating unit, and also a decasaccharide composed of a fragment of the O-specific polysaccharide chain and the core region, were obtained in minute amounts. On the basis of the sugar and methylation analyses, periodate oxidation, and {sup 1}H NMR spectroscopy of the lipopolysaccharide hydrolytic products, the biological repeating unit of the O-specific polysaccharide was shown to be a branched octasaccharide. The linkage between the O-specific polysaccharide chain and core region has also been determined and has yielded strong evidence that N-acetylneuraminic acid is an inherent lipopolysaccharide component. The lipopolysaccharide of H. alvei strain 2 is the first lipopolysaccharide reported to contain 4-substituted neuraminic acid in its O-specific polysaccharide region.

• OSTI ID: 5014341
• Journal Information: Biochemistry; (United States), Vol. 30:20; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
LIPOPOLYSACCHARIDES
MOLECULAR STRUCTURE
SIALIC ACID
NUCLEAR MAGNETIC RESONANCE
ACID HYDROLYSIS
BACTERIA
ELECTROPHORESIS
GAS CHROMATOGRAPHY
MASS SPECTROSCOPY
PROTONS
BARYONS
CARBOHYDRATES
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HYDROLYSIS
LIPIDS
LYSIS
MAGNETIC RESONANCE
MICROORGANISMS
MONOSACCHARIDES
NUCLEONS
ORGANIC COMPOUNDS
POLYSACCHARIDES
RESONANCE
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
SPECTROSCOPY
550601* - Medicine- Unsealed Radionuclides in Diagnostics