Complete structure of the cell surface polysaccharide of Streptococcus oralis ATCC 10557: A receptor for lectin-mediated interbacterial adherence

Journal Article · Tue Jul 02 00:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00240a025· OSTI ID:5013914

Abeygunawardana, C; Bush, C A ^[1]; Cisar, J O ^[2]

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1. Univ. of Maryland, Baltimore (United States)
2. National Inst. of Dental Research, Bethesda, MD (United States)

Lectin-carbohydrate binding is known to play an important role in a number of different cell-cell interactions including those between certain species of oral streptococci and actinomyces that colonize teeth. The cell wall polysaccharides of Streptococcus oralis ATCC 10557, S. oralis 34, and Streptococcus mitis J22, although not identical antigenically, each function as a receptor molecule for the galactose and N-acetylgalactosamine reactive fimbrial lectins of Actinomyces viscosus and Actinomyces naeslundii. Carbohydrate analysis of the receptor polysaccharide isolated from S. oralis ATCC 10557 shows galactose (3 mol), glucose (1 mol), GalNAc (1 mol), and rhamnose (1 mol). {sup 1}H NMR spectra of the polysaccharide show that is partially O-acetylated. Analysis of the {sup 1}H NMR spectrum of the de-O-acetylated polysaccharide shows that it is composed of repeating subunits containing six monosaccharides and that the subunits are joined by a phosphodiester linkage. The {sup 1}H and {sup 13}C NMR spectra were completely assigned by two-dimensional homonuclear correlation methods and by {sup 1}H-detected heteronuclear multiple-quantum correlation ({sup 1}H({sup 13}C)HMQC). The complete {sup 1}H and {sup 13}C assignment of the native polysaccharide was carried out by the same techniques augmented by a {sup 13}C-coupled hybrid HMQC-COSY method, which is shown to be especially useful for carbohydrates in which strong coupling and overlapping peaks in the {sup 1}H spectrum pose difficulties.

• OSTI ID: 5013914
• Journal Information: Biochemistry; (United States), Vol. 30:26; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
CELL WALL
MOLECULAR STRUCTURE
POLYSACCHARIDES
NUCLEAR MAGNETIC RESONANCE
ACETYLATION
ACTINOMYCES
CARBON 13
CHEMICAL SHIFT
LECTINS
PROTONS
RECEPTORS
STREPTOCOCCUS
ACYLATION
BACTERIA
BARYONS
CARBOHYDRATES
CARBON ISOTOPES
CELL CONSTITUENTS
CHEMICAL REACTIONS
ELEMENTARY PARTICLES
EVEN-ODD NUCLEI
FERMIONS
HADRONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MEMBRANE PROTEINS
MICROORGANISMS
NUCLEI
NUCLEONS
ORGANIC COMPOUNDS
PROTEINS
RESONANCE
SACCHARIDES
STABLE ISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics