Highly sensitive radioimmunoassay of atrial natriuretic peptide (ANP) in human plasma and urine

Journal Article · Thu May 29 00:00:00 EDT 1986 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(86)91200-3· OSTI ID:5009235

Marumo, F; Sakamoto, H; Ando, K; Ishigami, T; Kawakami, M

A highly sensitive radioimmunoassay has been established for measurement of human plasma and urine concentrations of atrial natriuretic peptide (ANP) and requires no extraction or concentration process. An antiserum was prepared from rabbits immunized with ..cap alpha..-human ANP (..cap alpha..-hANP) coupled with bovine-thyroglobulin. The sensitivity of this method was 0.2 pg/tube of synthetic ..cap alpha..-hANP utilized as authentic standard. Recovery of ..cap alpha..-hANP spiked to plasma and urine was 97.7 +/- 15.4% and 97.1 +/- 9.5% (mean +/- SD), respectively. Plasma and urinary ANP concentrations versus assay data showed satisfactory linearity. In 124 health subjects, the plasma ANP concentration was 31.7 +/- 12.0 pg/ml. Two different molecular forms of ANP in plasma and a single form in urine were found by gel permeation chromatography.

• Research Organization: Kitasato Univ. School of Medicine, Kanagawa, Japan
• OSTI ID: 5009235
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 137:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD PLASMA
RADIOIMMUNOASSAY
POLYPEPTIDES
URINE
CATTLE
EXPERIMENTAL DATA
GEL PERMEATION CHROMATOGRAPHY
IMMUNE SERUMS
MOLECULAR STRUCTURE
PATIENTS
RABBITS
SENSITIVITY
THYROGLOBULIN
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BODY FLUIDS
CHROMATOGRAPHY
DATA
DOMESTIC ANIMALS
GLOBULINS
IMMUNOASSAY
IMMUNOLOGY
INFORMATION
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
NUMERICAL DATA
ORGANIC COMPOUNDS
PEPTIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
RUMINANTS
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES
WASTES
550201* - Biochemistry- Tracer Techniques