Leukotriene B/sub 4/ production by stimulated whole blood: comparative studies with isolated polymorphonuclear cells
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
A new method was developed to study leukotriene B/sub 4/ (LTB/sub 4/) production by stimulated whole blood. The calcium ionophore A23187 and serum-treated zymosan induced LTB/sub 4/ production, measured by radioimmunoassay, in a dose- and time-dependent manner. The pattern of LTB/sub 4/ production by whole blood differed markedly from that observed with isolated, purified polymorphonuclear leukocytes. Higher levels of LTB/sub 4/ were reached and maintained in whole blood. The system allowed to detect drug effects on LTB/sub 4/ takes into account the complex interactions between different cell types which can modulate LTB/sub 4/ metabolism.
- Research Organization:
- Univ. of Leuven, Belgium
- OSTI ID:
- 5009133
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 137:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CARBOXYLIC ACIDS
BIOSYNTHESIS
RADIOIMMUNOASSAY
BIOCHEMICAL REACTION KINETICS
BLOOD
CALCIUM COMPOUNDS
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
IN VITRO
LEUKOCYTES
METABOLISM
TIME DEPENDENCE
ALKALINE EARTH METAL COMPOUNDS
BIOLOGICAL MATERIALS
BLOOD CELLS
BODY FLUIDS
DATA
IMMUNOASSAY
IMMUNOLOGY
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
MATERIALS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIOASSAY
RADIOIMMUNOLOGY
REACTION KINETICS
SYNTHESIS
TRACER TECHNIQUES
550201* - Biochemistry- Tracer Techniques
CARBOXYLIC ACIDS
BIOSYNTHESIS
RADIOIMMUNOASSAY
BIOCHEMICAL REACTION KINETICS
BLOOD
CALCIUM COMPOUNDS
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
IN VITRO
LEUKOCYTES
METABOLISM
TIME DEPENDENCE
ALKALINE EARTH METAL COMPOUNDS
BIOLOGICAL MATERIALS
BLOOD CELLS
BODY FLUIDS
DATA
IMMUNOASSAY
IMMUNOLOGY
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
MATERIALS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIOASSAY
RADIOIMMUNOLOGY
REACTION KINETICS
SYNTHESIS
TRACER TECHNIQUES
550201* - Biochemistry- Tracer Techniques