Differential generation of superoxide radical by rat phagocytes stimulated with IgA and IgG immune complexes
Rat glycogen elicited peritoneal neutrophils and bronchoalveolar lavage (BAL) marcophages were stimulated with preformed immune complexes consisting of monoclonal IgG anti-dinitorphenol linked bovine serum albumin (DNP-BSA) or monoclonal IgA anti-DNP-BSA. Superoxide (O/sub 2//sup tau/) was detected using superoxide dismutase inhibitable reduction of ferricytochrome C. Neutrophils (2 x 10/sup 6/) generated small (<2.3 nmole/30 min.) quantities of O/sub 2//sup tau/ when stimulated with IgA-DNP-BAS in the presence or absence of cytochalasin B (1.25 ..mu..g/ml). There was a 4 to 8-fold, dose-dependent increase in O/sub 2//sup tau/ generation by neutrophils stimulated with IgG-DNP-BSA. Cytochalasin B was required to generate this quantity (up to 7.7 nmol/30 min.) of O/sub 2//sup tau/. Whether stimulated with IgG-DNP-BSA or IgA-DNP-BSA, BAL macrophages (5 x 10/sup 5/) generated more O/sub 2//sup tau/ in the absence of cytochalasin B than in its presence. Increased O/sub 2//sup tau/ generation by the immune complex stimulated BAL macrophages was dose-dependent and increased to 6-fold with IgG-DNP-BSA stimulated cells and up to 4-fold with IgA-DNP-BSA stimulated cells. These data corroborate published in vivo studies suggesting that IgA immune complex mediated tissue injury may be mediated by macrophages and not neutrophils. These data also suggest that the mechanisms for immune complex induced O/sub 2//sup tau/ generation may vary depending both on the phagocyte as well as on the stimulus.
- Research Organization:
- Univ. of Michigan Medical School, Ann Arbor
- OSTI ID:
- 5006974
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Preferential enhancement of reverse dermal Arthus reaction by polyelectrolytes: in vivo and in vitro evidence for mediation by oxygen-derived radicals and their metabolites
Evidence for immunoglobulin Fc receptor-mediated prostaglandin2 and platelet-activating factor formation by cultured rat mesangial cells
Related Subjects
IMMUNOGLOBULINS
BIOLOGICAL EFFECTS
SUPEROXIDE RADICALS
BIOSYNTHESIS
ALBUMINS
DOSE-RESPONSE RELATIONSHIPS
IMMUNE REACTIONS
LAVAGE
MACROPHAGES
MONOCLONAL ANTIBODIES
NEUTROPHILS
PHAGOCYTES
PHAGOCYTOSIS
RATS
SUPEROXIDE DISMUTASE
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ENZYMES
GLOBULINS
LEUKOCYTES
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
RADICALS
RODENTS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
550900* - Pathology