Effect of a high molecular weight hyaluronic acid (HA) preparation on the stimulation of polymorphonulcear leukocytes (PMNL)
During the process of joint inflammation PMNL are attracted into the joint space by chemotactic agents and are stimulated by immune complexes, particular matter (eg, crystals, cartilage debris) and other phlogistic agents. This process occurs in an environment rich in HA. The authors have examined the effect of high molecular weight HA. They have examined the effect of high molecular weight HA upon PMNL stimulation. PMNL were isolated from human blood and stimulated with either opsonized zymosan or formyl-methionyl-leucyl-phenylalanine (fmlp). The authors assessed stimulation by measuring the ability of cell supernatants to promote the release of /sup 35/S from chips of rabbit articular cartilage labeled in vivo, and the enhancement of oxidation of (1-/sup 14/C)glucose to /sup 14/CO/sub 2/. Stimulation of cells with zym in the presence of HA, 0.125-2.5 mg/ml, resulted in enhanced /sup 35/S release (33-59% over zym alone) and /sup 14/CO/sub 2/ production (0.5-64%). However, HA failed to enhance responses when fmlp (+cytochalasin B) was used as the stimulus. It has been demonstrated that high molecular weight HA inhibits phagocytosis of both latex and aggregated IgG. In our studies, it is likely that HA interference with ingestion of zym leads to frustrated phagocytosis and enhancement of PMNL responses. Similar modification of responses of inflammatory mediator cells could occur in inflamed joints.
- Research Organization:
- Univ. of Illinois College of Medicine, Chicago
- OSTI ID:
- 5006414
- Report Number(s):
- CONF-8604222-; TRN: 86-037032
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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BONE JOINTS
INFLAMMATION
HYALURONIC ACID
BIOLOGICAL EFFECTS
LEUKOCYTES
PHAGOCYTOSIS
CARBON 14 COMPOUNDS
CARBON DIOXIDE
GLUCOSE
IMMUNE REACTIONS
MOLECULAR WEIGHT
SULFUR 35
TRACER TECHNIQUES
ALDEHYDES
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BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
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BLOOD CELLS
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CHALCOGENIDES
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
HEXOSES
ISOTOPE APPLICATIONS
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LABELLED COMPOUNDS
LIGHT NUCLEI
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MONOSACCHARIDES
MUCOPOLYSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
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550901* - Pathology- Tracer Techniques