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Title: Iodine-125-labeled lipoprotein lipase as a tool to detect and study spontaneous lipolysis in bovine milk

Abstract

The distribution of lipoprotein lipase among cream, casein, and milk serum can be evaluated by addition of a trace amount of /sup 125/I-labeled lipoprotein lipase to milk. Radioactive lipase was distributed in parallel to endogenous lipase under several conditions. In some milk samples, binding of lipase to cream increased when the milk was cooled. Correlation was good between bound labeled lipase and degree of cold-induced lipolysis in corresponding milk samples. Binding of lipase to cream or to casein was not saturable by addition of two-to threefold more lipase than is normally present in milk. In milk with a relatively high fraction of lipase bound to cream, a correspondingly lower fraction was associated with casein, whereas the fraction of lipase in milk serum was similar in all milk samples. Cold-induced binding of lipoprotein lipase to cream was not fully reversed when the milk was warmed again. Heparin released lipase from casein and increased the amount of lipase bound to cream after cooling.

Authors:
;
Publication Date:
Research Org.:
Agricultural Univ. of Norway
OSTI Identifier:
5006351
Resource Type:
Journal Article
Journal Name:
J. Dairy Sci.; (United States)
Additional Journal Information:
Journal Volume: 7
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; LIPOPROTEINS; LYSIS; CATTLE; IODINE 125; LABELLED COMPOUNDS; LIPASE; MILK; TRACER TECHNIQUES; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BODY FLUIDS; CARBOXYLESTERASES; DAYS LIVING RADIOISOTOPES; DOMESTIC ANIMALS; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; ESTERASES; FOOD; HYDROLASES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LIPIDS; MAMMALS; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PROTEINS; RADIOISOTOPES; RUMINANTS; VERTEBRATES; 553001* - Agriculture & Food Technology- Tracer Techniques- (-1987)

Citation Formats

Sundheim, G, and Bengtsson-Olivecrona, G. Iodine-125-labeled lipoprotein lipase as a tool to detect and study spontaneous lipolysis in bovine milk. United States: N. p., 1986. Web. doi:10.3168/jds.S0022-0302(86)80600-2.
Sundheim, G, & Bengtsson-Olivecrona, G. Iodine-125-labeled lipoprotein lipase as a tool to detect and study spontaneous lipolysis in bovine milk. United States. https://doi.org/10.3168/jds.S0022-0302(86)80600-2
Sundheim, G, and Bengtsson-Olivecrona, G. 1986. "Iodine-125-labeled lipoprotein lipase as a tool to detect and study spontaneous lipolysis in bovine milk". United States. https://doi.org/10.3168/jds.S0022-0302(86)80600-2.
@article{osti_5006351,
title = {Iodine-125-labeled lipoprotein lipase as a tool to detect and study spontaneous lipolysis in bovine milk},
author = {Sundheim, G and Bengtsson-Olivecrona, G},
abstractNote = {The distribution of lipoprotein lipase among cream, casein, and milk serum can be evaluated by addition of a trace amount of /sup 125/I-labeled lipoprotein lipase to milk. Radioactive lipase was distributed in parallel to endogenous lipase under several conditions. In some milk samples, binding of lipase to cream increased when the milk was cooled. Correlation was good between bound labeled lipase and degree of cold-induced lipolysis in corresponding milk samples. Binding of lipase to cream or to casein was not saturable by addition of two-to threefold more lipase than is normally present in milk. In milk with a relatively high fraction of lipase bound to cream, a correspondingly lower fraction was associated with casein, whereas the fraction of lipase in milk serum was similar in all milk samples. Cold-induced binding of lipoprotein lipase to cream was not fully reversed when the milk was warmed again. Heparin released lipase from casein and increased the amount of lipase bound to cream after cooling.},
doi = {10.3168/jds.S0022-0302(86)80600-2},
url = {https://www.osti.gov/biblio/5006351}, journal = {J. Dairy Sci.; (United States)},
number = ,
volume = 7,
place = {United States},
year = {Tue Jul 01 00:00:00 EDT 1986},
month = {Tue Jul 01 00:00:00 EDT 1986}
}