Increased photosensitivity to near-ultraviolet light in murine SLE
The authors investigated whether there is increased susceptibility to near-UVL in murine SLE. Cultured spleen cells from either strain of mice with lupus disease or conventional strains of mice were exposed to different UVL fractions in vitro. The effect of DNA synthesis, release, and repair was examined. DNA synthesis and release was measured as percent of (/sup 3/H)thymidine (dT) uptake into either total acid-precipitable radioactive material of cell sediment plus supernatant, or that of the medium alone, whereas hydroxyurea-resistant dT incorporation represented DNA repair. The data indicate that all SLE strains, in contrast to all non-SLE strains, show increased DNA synthesis and release after UV-A exposure. In addition, all murine SLE strains demonstrate increased susceptibility to induction of DNA damage by UV-A. The significance of these observations in relation to the clinical activity of SLE after sunlight exposure is discussed.
- Research Organization:
- Harvard Medical School, Boston, MA
- OSTI ID:
- 5004022
- Journal Information:
- J. Immunol.; (United States), Vol. 132:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA REPAIR
BIOLOGICAL RADIATION EFFECTS
DNA REPLICATION
MICE
RADIOSENSITIVITY
LUPUS
NEAR ULTRAVIOLET RADIATION
SPLEEN CELLS
THYMIDINE
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AZINES
BACTERIAL DISEASES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DISEASES
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
INFECTIOUS DISEASES
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
RODENTS
SKIN DISEASES
SOMATIC CELLS
TUBERCULOSIS
ULTRAVIOLET RADIATION
VERTEBRATES
560152* - Radiation Effects on Animals- Animals