Activation of phosphatidic acid metabolism of human erythrocyte membranes by perfringolysin O

Journal Article · Thu May 29 00:00:00 EDT 1986 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(86)91170-8· OSTI ID:5003087

Saito, M; Ando, S; Mitsui, K; Homma, Y; Takenawa, T

The effect of perfringolysin O on the lipid metabolism of human erythrocyte membranes was investigated. Erythrocytes were prelabeled with (/sup 3/H)arachidonic acid and (/sup 32/P)inorganic phosphate. In the presence of calcium ion (5.5 mM), the effect of perfringolysin O on lipid metabolism was very similar to that of an calcium-ionophore A23187. In the absence of calcium ion, the accumulation of phosphatidic acid and its following decreasing trend were observed during the reaction with the toxin. Such changes were not caused by filipin. These results suggest that perfringolysin O causes the activation of a diglyceride-phosphatidic acid cycle, which might be involved in the calcium transport.

• Research Organization: Tokyo Metropolitan Institute of Gerontology, Japan
• OSTI ID: 5003087
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 137:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
LIPIDS
BIOLOGICAL ACCUMULATION
METABOLISM
TOXINS
TOXICITY
ARACHIDONIC ACID
CALCIUM COMPOUNDS
CATIONS
CELL MEMBRANES
CHEMICAL ACTIVATION
ERYTHROCYTES
EXPERIMENTAL DATA
MEMBRANE TRANSPORT
PHOSPHATES
PHOSPHORUS 32
RESPONSE MODIFYING FACTORS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHARGED PARTICLES
DATA
DAYS LIVING RADIOISOTOPES
INFORMATION
IONS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MATERIALS
MEMBRANES
MONOCARBOXYLIC ACIDS
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
TOXIC MATERIALS
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550201 - Biochemistry- Tracer Techniques