In vitro hydrogen production by glucose dehydrogenase and hydrogenase
Abstract
A new in vitro enzymatic pathway for the generation of molecular hydrogen from glucose has been demonstrated. The reaction is based on the oxidation of glucose by Thermoplasma acidophilum glucose dehydrogenase with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase. Stoichiometric yields of hydrogen were produced from glucose with the continuous recycling of cofactor. This simple system may provide a method for the biological production of hydrogen from renewable sources. In addition, the other product of this reaction, gluconic acid, is a high-value chemical commodity. 23 refs., 5 figs.
- Authors:
-
- Oak Ridge National Lab., TN (United States)
- Univ. of Bath (United Kingdom); and others
- Publication Date:
- OSTI Identifier:
- 495272
- DOE Contract Number:
- AC05-96OR22464
- Resource Type:
- Journal Article
- Journal Name:
- Bio/Technology
- Additional Journal Information:
- Journal Volume: 14; Journal Issue: 7; Other Information: PBD: Jul 1996
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; OXIDOREDUCTASES; ENZYME ACTIVITY; HYDROGENASES; GLUCOSE; OXIDATION; INTERSTITIAL HYDROGEN GENERATION; EVALUATION; OPTIMIZATION; BIOLOGICAL PATHWAYS; DESIGN; RENEWABLE ENERGY SOURCES; GLUCONIC ACID; PRODUCTION; GENES; GENE REGULATION; DNA SEQUENCING; DNA-CLONING; HYDROGEN; ORNL; BIOMASS; NADP; BACTERIA; STOICHIOMETRY
Citation Formats
Woodward, J, Mattingly, S M, and Danson, M. In vitro hydrogen production by glucose dehydrogenase and hydrogenase. United States: N. p., 1996.
Web. doi:10.1038/nbt0796-872.
Woodward, J, Mattingly, S M, & Danson, M. In vitro hydrogen production by glucose dehydrogenase and hydrogenase. United States. https://doi.org/10.1038/nbt0796-872
Woodward, J, Mattingly, S M, and Danson, M. 1996.
"In vitro hydrogen production by glucose dehydrogenase and hydrogenase". United States. https://doi.org/10.1038/nbt0796-872.
@article{osti_495272,
title = {In vitro hydrogen production by glucose dehydrogenase and hydrogenase},
author = {Woodward, J and Mattingly, S M and Danson, M},
abstractNote = {A new in vitro enzymatic pathway for the generation of molecular hydrogen from glucose has been demonstrated. The reaction is based on the oxidation of glucose by Thermoplasma acidophilum glucose dehydrogenase with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase. Stoichiometric yields of hydrogen were produced from glucose with the continuous recycling of cofactor. This simple system may provide a method for the biological production of hydrogen from renewable sources. In addition, the other product of this reaction, gluconic acid, is a high-value chemical commodity. 23 refs., 5 figs.},
doi = {10.1038/nbt0796-872},
url = {https://www.osti.gov/biblio/495272},
journal = {Bio/Technology},
number = 7,
volume = 14,
place = {United States},
year = {Mon Jul 01 00:00:00 EDT 1996},
month = {Mon Jul 01 00:00:00 EDT 1996}
}
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