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Title: In vitro hydrogen production by glucose dehydrogenase and hydrogenase

Abstract

A new in vitro enzymatic pathway for the generation of molecular hydrogen from glucose has been demonstrated. The reaction is based on the oxidation of glucose by Thermoplasma acidophilum glucose dehydrogenase with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase. Stoichiometric yields of hydrogen were produced from glucose with the continuous recycling of cofactor. This simple system may provide a method for the biological production of hydrogen from renewable sources. In addition, the other product of this reaction, gluconic acid, is a high-value chemical commodity. 23 refs., 5 figs.

Authors:
;  [1];  [2]
  1. Oak Ridge National Lab., TN (United States)
  2. Univ. of Bath (United Kingdom); and others
Publication Date:
OSTI Identifier:
495272
DOE Contract Number:  
AC05-96OR22464
Resource Type:
Journal Article
Journal Name:
Bio/Technology
Additional Journal Information:
Journal Volume: 14; Journal Issue: 7; Other Information: PBD: Jul 1996
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; OXIDOREDUCTASES; ENZYME ACTIVITY; HYDROGENASES; GLUCOSE; OXIDATION; INTERSTITIAL HYDROGEN GENERATION; EVALUATION; OPTIMIZATION; BIOLOGICAL PATHWAYS; DESIGN; RENEWABLE ENERGY SOURCES; GLUCONIC ACID; PRODUCTION; GENES; GENE REGULATION; DNA SEQUENCING; DNA-CLONING; HYDROGEN; ORNL; BIOMASS; NADP; BACTERIA; STOICHIOMETRY

Citation Formats

Woodward, J, Mattingly, S M, and Danson, M. In vitro hydrogen production by glucose dehydrogenase and hydrogenase. United States: N. p., 1996. Web. doi:10.1038/nbt0796-872.
Woodward, J, Mattingly, S M, & Danson, M. In vitro hydrogen production by glucose dehydrogenase and hydrogenase. United States. https://doi.org/10.1038/nbt0796-872
Woodward, J, Mattingly, S M, and Danson, M. 1996. "In vitro hydrogen production by glucose dehydrogenase and hydrogenase". United States. https://doi.org/10.1038/nbt0796-872.
@article{osti_495272,
title = {In vitro hydrogen production by glucose dehydrogenase and hydrogenase},
author = {Woodward, J and Mattingly, S M and Danson, M},
abstractNote = {A new in vitro enzymatic pathway for the generation of molecular hydrogen from glucose has been demonstrated. The reaction is based on the oxidation of glucose by Thermoplasma acidophilum glucose dehydrogenase with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase. Stoichiometric yields of hydrogen were produced from glucose with the continuous recycling of cofactor. This simple system may provide a method for the biological production of hydrogen from renewable sources. In addition, the other product of this reaction, gluconic acid, is a high-value chemical commodity. 23 refs., 5 figs.},
doi = {10.1038/nbt0796-872},
url = {https://www.osti.gov/biblio/495272}, journal = {Bio/Technology},
number = 7,
volume = 14,
place = {United States},
year = {Mon Jul 01 00:00:00 EDT 1996},
month = {Mon Jul 01 00:00:00 EDT 1996}
}