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Title: Phthalate biotransformation by rainbow trout

Abstract

The biotransformation of di(2-ethylhexyl)phthalate (DEHP) was studied in rainbow trout because DEHP bioconcentration is limited by metabolism. Biological fluids were collected following intravascular administration. Methylesterified metabolites were identified using rodent-derived standards and nonlinear gradient elution HPLC; metabolites were confirmed by gas chromatography. Similarities between the biotransformation of DEHP by rainbow trout and mammalian species included: (1) mono-2-ethylhexyl phthalate (MEHP) appeared to be the obligatory first step in DEHP metabolism; (2) the phthalate ring was not oxidized; (3) phthalic acid was a minor metabolite; and (4) several metabolites contained multiple oxidations of the 2-ethylhexyl moiety of MEHP. No metabolites unique to rainbow trout were identified. However, fewer oxidized metabolites were identified in rainbow trout than in mammalian species, possibly due to limited mitochondrial metabolism of MEHP in rainbow trout. The amount of biliary MEHP glucuronide after intravascular administration of DEHP was substantially less than reported in rainbow trout exposed to DEHP via the water. The results confirmed that DEHP metabolism in rainbow trout proceeds by initial rapid formation of MEHP, followed by excretion or extensive oxidation by microsomal P450.

Authors:
 [1];  [2];  [3]
  1. RCG/Hagler Bailly, Boulder, CO (United States)
  2. NIEHS, Research Triangle Park, NC (United States)
  3. Ohio State Univ., Columbus, OH (United States)
Publication Date:
OSTI Identifier:
49470
Report Number(s):
CONF-9410273-
TRN: IM9523%%293
Resource Type:
Conference
Resource Relation:
Conference: 15. annual meeting of the Society of Environmental Toxicology and Chemistry (SETAC), Denver, CO (United States), 30 Oct - 3 Nov 1994; Other Information: PBD: 1994; Related Information: Is Part Of Society of Environmental Toxicology and Chemistry 15th annual meeting: Abstract book. Ecological risk: Science, policy, law, and perception; PB: 286 p.
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; TROUT; METABOLISM; PHTHALATES; WATER POLLUTION; MICROSOMES; MIXED-FUNCTION OXIDASES

Citation Formats

Barron, M G, Albro, P W, and Hayton, W L. Phthalate biotransformation by rainbow trout. United States: N. p., 1994. Web.
Barron, M G, Albro, P W, & Hayton, W L. Phthalate biotransformation by rainbow trout. United States.
Barron, M G, Albro, P W, and Hayton, W L. 1994. "Phthalate biotransformation by rainbow trout". United States.
@article{osti_49470,
title = {Phthalate biotransformation by rainbow trout},
author = {Barron, M G and Albro, P W and Hayton, W L},
abstractNote = {The biotransformation of di(2-ethylhexyl)phthalate (DEHP) was studied in rainbow trout because DEHP bioconcentration is limited by metabolism. Biological fluids were collected following intravascular administration. Methylesterified metabolites were identified using rodent-derived standards and nonlinear gradient elution HPLC; metabolites were confirmed by gas chromatography. Similarities between the biotransformation of DEHP by rainbow trout and mammalian species included: (1) mono-2-ethylhexyl phthalate (MEHP) appeared to be the obligatory first step in DEHP metabolism; (2) the phthalate ring was not oxidized; (3) phthalic acid was a minor metabolite; and (4) several metabolites contained multiple oxidations of the 2-ethylhexyl moiety of MEHP. No metabolites unique to rainbow trout were identified. However, fewer oxidized metabolites were identified in rainbow trout than in mammalian species, possibly due to limited mitochondrial metabolism of MEHP in rainbow trout. The amount of biliary MEHP glucuronide after intravascular administration of DEHP was substantially less than reported in rainbow trout exposed to DEHP via the water. The results confirmed that DEHP metabolism in rainbow trout proceeds by initial rapid formation of MEHP, followed by excretion or extensive oxidation by microsomal P450.},
doi = {},
url = {https://www.osti.gov/biblio/49470}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Dec 31 00:00:00 EST 1994},
month = {Sat Dec 31 00:00:00 EST 1994}
}

Conference:
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