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Title: Direct inhibition of plant mitochondrial respiration by elevated CO{sub 2}

Abstract

Doubling the concentration of atmospheric CO{sub 2} often inhibits plant respiration, but the mechanistic basis of this effect is unknown. We investigated the direct effects of increasing the concentration of CO{sub 2} by 360 {mu}L L{sup -1} above ambient on O{sub 2} uptake in isolated mitochondria from soybean (Glycine max L. cv Ransom) cotyledons. Increasing the CO{sub 2} concentration inhibited the oxidation of succinate, external NADH, and succinate and external NADH combined. The inhibition was greater when mitochondria were preincubated for 10 min in the presence of the elevated CO{sub 2} concentration inhibited the salicylhydroxamic acid-resistant cytochrome pathway. We also investigated the direct effects of elevated CO{sub 2} concentration on the activities of cytochrome c oxidase and succinate dehydrogenase (SDH) and found that the activity of both enzymes was inhibited. The kinetics of inhibition of cytochrome c oxidase were time-dependent. The level of SDH inhibition depended on the concentration of succinate in the reaction mixture. Direct inhibition of respiration by elevated CO{sub 2} in plants and intact tissues may be due at least in part to the inhibition of cytochrome c oxidase and SDH. 42 refs., 5 figs., 1 tab.

Authors:
;  [1]; ;  [2]
  1. Smithsonian Environmental Research Center, Edgewater, MD (United States)
  2. Duke Univ., Durham, NC (United States)
Publication Date:
OSTI Identifier:
494257
Resource Type:
Journal Article
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Volume: 112; Journal Issue: 3; Other Information: PBD: Nov 1996
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; CARBON DIOXIDE; BIOLOGICAL EFFECTS; SOYBEANS; RESPIRATION; MITOCHONDRIA; OXIDATION; PLANTS; UPTAKE

Citation Formats

Gonzalez-Meler, M A, Drake, B G, Ribas-Carbo, M, and Siedow, J N. Direct inhibition of plant mitochondrial respiration by elevated CO{sub 2}. United States: N. p., 1996. Web.
Gonzalez-Meler, M A, Drake, B G, Ribas-Carbo, M, & Siedow, J N. Direct inhibition of plant mitochondrial respiration by elevated CO{sub 2}. United States.
Gonzalez-Meler, M A, Drake, B G, Ribas-Carbo, M, and Siedow, J N. 1996. "Direct inhibition of plant mitochondrial respiration by elevated CO{sub 2}". United States.
@article{osti_494257,
title = {Direct inhibition of plant mitochondrial respiration by elevated CO{sub 2}},
author = {Gonzalez-Meler, M A and Drake, B G and Ribas-Carbo, M and Siedow, J N},
abstractNote = {Doubling the concentration of atmospheric CO{sub 2} often inhibits plant respiration, but the mechanistic basis of this effect is unknown. We investigated the direct effects of increasing the concentration of CO{sub 2} by 360 {mu}L L{sup -1} above ambient on O{sub 2} uptake in isolated mitochondria from soybean (Glycine max L. cv Ransom) cotyledons. Increasing the CO{sub 2} concentration inhibited the oxidation of succinate, external NADH, and succinate and external NADH combined. The inhibition was greater when mitochondria were preincubated for 10 min in the presence of the elevated CO{sub 2} concentration inhibited the salicylhydroxamic acid-resistant cytochrome pathway. We also investigated the direct effects of elevated CO{sub 2} concentration on the activities of cytochrome c oxidase and succinate dehydrogenase (SDH) and found that the activity of both enzymes was inhibited. The kinetics of inhibition of cytochrome c oxidase were time-dependent. The level of SDH inhibition depended on the concentration of succinate in the reaction mixture. Direct inhibition of respiration by elevated CO{sub 2} in plants and intact tissues may be due at least in part to the inhibition of cytochrome c oxidase and SDH. 42 refs., 5 figs., 1 tab.},
doi = {},
url = {https://www.osti.gov/biblio/494257}, journal = {Plant Physiology (Bethesda)},
number = 3,
volume = 112,
place = {United States},
year = {Fri Nov 01 00:00:00 EST 1996},
month = {Fri Nov 01 00:00:00 EST 1996}
}