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Title: Nutrient-stimulated biodegradation of aged refinery hydrocarbons in soil

Abstract

Aged hydrocarbon-contaminated refinery soil was amended with water and nutrients and tilled weekly for 1 year to stimulate biodegradation. Gas chromatography/mass spectrometry (GC/MS) analysis of polycyclic aromatic compounds (PAHs) and triterpane biomarkers, and Freon IR analysis of total petroleum hydrocarbons (TPH), were used to determine the extent of biodegradation. There was significant degradation of extractable hydrocarbon (up to 60%), but neither hopane, oleanane, nor the amount of polars decreased during this period of bioremediation, allowing them to be used as conserved internal markers for estimating biodegradation. Significant degradation of the more alkylated two- and three-ring compounds, and of the four-ring species pyrene and chrysene and their alkylated congeners, was seen. Substantial degradation (> 40%) of benzo(b)fluoranthene, benzo(k)fluoranthene, and benzo(a)pyrene also was seen. The results show that bioremediation can be a useful treatment in the cleanup of contaminated refinery sites.

Authors:
; ; ; ; ;  [1];  [2]
  1. Exxon Research and Engineering, Annandale, NJ (United States)
  2. Arthur D. Little, Inc., Cambridge, MA (United States)
Publication Date:
OSTI Identifier:
492229
Report Number(s):
CONF-950483-
ISBN 1-57477-006-3; TRN: 97:011907
Resource Type:
Conference
Resource Relation:
Conference: 3. international in situ and on-site bioreclamation symposium, San Diego, CA (United States), 24-27 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of Monitoring and verification of bioremediation; Hinchee, R.E. [ed.] [Battelle Memorial Inst., Columbus, OH (United States)]; Douglas, G.S. [ed.] [Arthur D. Little, Inc., Cambridge, MA (United States)]; Ong, S.K. [ed.] [Iowa State Univ., Ames, IA (United States)]; PB: 283 p.; Bioremediation, Volume 3(5)
Country of Publication:
United States
Language:
English
Subject:
02 PETROLEUM; BIODEGRADATION; REMEDIAL ACTION; OIL SPILLS; HYDROCARBONS; SOILS; PETROLEUM REFINERIES; ENVIRONMENTAL IMPACTS; GAS CHROMATOGRAPHY; MASS SPECTROSCOPY; NUTRIENTS

Citation Formats

Drake, E N, Stokley, K E, Calcavecchio, P, Bare, R E, Rothenburger, S J, Prince, R C, and Douglas, G S. Nutrient-stimulated biodegradation of aged refinery hydrocarbons in soil. United States: N. p., 1995. Web.
Drake, E N, Stokley, K E, Calcavecchio, P, Bare, R E, Rothenburger, S J, Prince, R C, & Douglas, G S. Nutrient-stimulated biodegradation of aged refinery hydrocarbons in soil. United States.
Drake, E N, Stokley, K E, Calcavecchio, P, Bare, R E, Rothenburger, S J, Prince, R C, and Douglas, G S. 1995. "Nutrient-stimulated biodegradation of aged refinery hydrocarbons in soil". United States.
@article{osti_492229,
title = {Nutrient-stimulated biodegradation of aged refinery hydrocarbons in soil},
author = {Drake, E N and Stokley, K E and Calcavecchio, P and Bare, R E and Rothenburger, S J and Prince, R C and Douglas, G S},
abstractNote = {Aged hydrocarbon-contaminated refinery soil was amended with water and nutrients and tilled weekly for 1 year to stimulate biodegradation. Gas chromatography/mass spectrometry (GC/MS) analysis of polycyclic aromatic compounds (PAHs) and triterpane biomarkers, and Freon IR analysis of total petroleum hydrocarbons (TPH), were used to determine the extent of biodegradation. There was significant degradation of extractable hydrocarbon (up to 60%), but neither hopane, oleanane, nor the amount of polars decreased during this period of bioremediation, allowing them to be used as conserved internal markers for estimating biodegradation. Significant degradation of the more alkylated two- and three-ring compounds, and of the four-ring species pyrene and chrysene and their alkylated congeners, was seen. Substantial degradation (> 40%) of benzo(b)fluoranthene, benzo(k)fluoranthene, and benzo(a)pyrene also was seen. The results show that bioremediation can be a useful treatment in the cleanup of contaminated refinery sites.},
doi = {},
url = {https://www.osti.gov/biblio/492229}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Dec 31 00:00:00 EST 1995},
month = {Sun Dec 31 00:00:00 EST 1995}
}

Conference:
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