Assessing bioremediation of crude oil in soils and sludges
- Exxon Production Research Co., Houston, TX (United States)
- American Chemical Society, Washington, DC (United States)
- Texas A and M Univ., College Station, TX (United States). Geochemical and Environmental Research Group
- Arthur D. Little, Inc., Cambridge, MA (United States)
Standard bulk property analytical methods currently being employed to evaluate crude oil bioremediation efficacy in soils provide no information concerning the mechanisms by which hydrocarbon losses are occurring (e.g., biodegradation versus leaching). Site/sample heterogeneity in field bioremediation projects may make it difficult to accurately quantify hydrocarbon losses due to biodegradation. To better understand the mechanisms by which losses are occurring and to accurately evaluate biodegradation rates, the hydrocarbon analytical methods must provide both quantitative and compositional information. In this study laboratory bioremediation experiments were used to compare the results of bulk property analytical methods with those methods used by petroleum geochemists that provide both quantitative and compositional data. A tecator extraction was used to isolate the total extractable matter (TEM) from the samples. Compositional changes were monitored by (1) column chromatography to determine class distributions, (2) high resolution gas chromatography with a flame-ionization detector (GC/FID) and (3) gas chromatography/mass spectrometry (GC/MS). Illustrations of the compositional changes detected by each method and their application to validating bioremediation are provided.
- OSTI ID:
- 492227
- Report Number(s):
- CONF-950483-; ISBN 1-57477-006-3; TRN: 97:011905
- Resource Relation:
- Conference: 3. international in situ and on-site bioreclamation symposium, San Diego, CA (United States), 24-27 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of Monitoring and verification of bioremediation; Hinchee, R.E. [ed.] [Battelle Memorial Inst., Columbus, OH (United States)]; Douglas, G.S. [ed.] [Arthur D. Little, Inc., Cambridge, MA (United States)]; Ong, S.K. [ed.] [Iowa State Univ., Ames, IA (United States)]; PB: 283 p.; Bioremediation, Volume 3(5)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Comparison of analytical methods used to measure petroleum hydrocarbons in soils and their applications to bioremediation
Field test for treatment verification of an in-situ enhanced bioremediation study