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Title: Microbial ecology and transformations associated with munitions contaminated soils

Abstract

Many acres of soil at the former Nebraska Ordnance Plant (NOP) are contaminated with TNT and other munitions residues. In some areas, solid phase TNT is present and controls the concentration of the soil solution. Native microbial populations in uncontaminated soils similar to those at the NOP site were severely reduced when solid phase TNT was allowed to control the soil solution TNT concentration. However, examination of NOP soil revealed an active population of Pseudomonas sp. A single species that could utilize TNT as a sole C source was isolated from the contaminated soil and tentatively identified as Pseudomonas corrugata through the BIOLOG system. Subsequent growth and characterization experiments indicate that the Pseudomonad metabolizes TNT while in the exponential phase of growth in medium containing glucose as a sole N source. Low TNT mineralization rates (measured by CO{sub 2} evolution) in soil and media using the various isolates suggest reduced availability due to sorption and incorporation of transformation intermediates into the organic matrix and microbial biomass. Pretreatment of TNT by acid-metal catalyzed reduction resulted in an initially higher rate of mineralization following addition to TNT-contaminated soil. Observations indicate more rapid microbial utilization of the 2,4,6-triaminotoluene (TAT) reduction product and itsmore » spontaneous decay product, methylphloroglucinol (2,4,6-trihydroxytoluene), than TNT. Abiotic pretreatment may be useful in enhancing microbial transformation and detoxification of TNT in highly contaminated soils.« less

Authors:
; ; ; ;  [1]
  1. Univ. of Nebraska, Lincoln, NE (United States)
Publication Date:
OSTI Identifier:
491074
Report Number(s):
CONF-9406250-
TRN: IM9729%%325
Resource Type:
Conference
Resource Relation:
Conference: 9. Annual conference on hazardous waste remediation, Bozeman, MT (United States), 8-10 Jun 1994; Other Information: PBD: 1994; Related Information: Is Part Of Proceedings of the 9. annual conference on hazardous waste remediation; Erickson, L.E.; Tillison, D.L.; Grant, S.C.; McDonald, J.P. [eds.]; PB: 389 p.
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE; NITRO COMPOUNDS; REMEDIAL ACTION; BIODEGRADATION; SOILS; NEBRASKA; PSEUDOMONAS; TNT

Citation Formats

Martin, J L, Li, Z, Kokjohn, T A, Shea, P J, and Comfort, S D. Microbial ecology and transformations associated with munitions contaminated soils. United States: N. p., 1994. Web.
Martin, J L, Li, Z, Kokjohn, T A, Shea, P J, & Comfort, S D. Microbial ecology and transformations associated with munitions contaminated soils. United States.
Martin, J L, Li, Z, Kokjohn, T A, Shea, P J, and Comfort, S D. 1994. "Microbial ecology and transformations associated with munitions contaminated soils". United States.
@article{osti_491074,
title = {Microbial ecology and transformations associated with munitions contaminated soils},
author = {Martin, J L and Li, Z and Kokjohn, T A and Shea, P J and Comfort, S D},
abstractNote = {Many acres of soil at the former Nebraska Ordnance Plant (NOP) are contaminated with TNT and other munitions residues. In some areas, solid phase TNT is present and controls the concentration of the soil solution. Native microbial populations in uncontaminated soils similar to those at the NOP site were severely reduced when solid phase TNT was allowed to control the soil solution TNT concentration. However, examination of NOP soil revealed an active population of Pseudomonas sp. A single species that could utilize TNT as a sole C source was isolated from the contaminated soil and tentatively identified as Pseudomonas corrugata through the BIOLOG system. Subsequent growth and characterization experiments indicate that the Pseudomonad metabolizes TNT while in the exponential phase of growth in medium containing glucose as a sole N source. Low TNT mineralization rates (measured by CO{sub 2} evolution) in soil and media using the various isolates suggest reduced availability due to sorption and incorporation of transformation intermediates into the organic matrix and microbial biomass. Pretreatment of TNT by acid-metal catalyzed reduction resulted in an initially higher rate of mineralization following addition to TNT-contaminated soil. Observations indicate more rapid microbial utilization of the 2,4,6-triaminotoluene (TAT) reduction product and its spontaneous decay product, methylphloroglucinol (2,4,6-trihydroxytoluene), than TNT. Abiotic pretreatment may be useful in enhancing microbial transformation and detoxification of TNT in highly contaminated soils.},
doi = {},
url = {https://www.osti.gov/biblio/491074}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Dec 31 00:00:00 EST 1994},
month = {Sat Dec 31 00:00:00 EST 1994}
}

Conference:
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