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Title: Effect of heavy metals on ortho-nitrophenol biodegradation by Pseudomonas aeruginosa B{sub 8}

Conference ·
OSTI ID:490981
;  [1]
  1. Sofia Univ. (Bulgaria). Faculty of Biology

The ortho-nitrophenol (ONP)-degrading strain of Pseudomonas aeruginosa B{sub 8}, treated by the high selective pressure method toward high xenobiotic concentrations, shows wide substrate specificity toward phenol, ONP, 2,4-dinitrophenol (2,4-DNP), 2,5-DNP, 2,6-DNP, and pentachlorophenol (PCP). The ONP biodegradation activity of strain B{sub 8}, and biomass accumulation when ONP in a concentration of 0.29 mMol was the sole carbon and energy source, have been investigated. The influence of different heavy metals (Cu{sup 2+}, Fe{sup 2+}, Fe{sup 3+}, Pb{sup 2+}, and the combination Fe{sup 3+}/Pb{sup 2+}) in a concentration of 40 mg/L on ONP biodegradation, biomass accumulation, and oxygenase activities by whole cells and in cell extracts was studied comparatively. Cu{sup 2+} and Pb{sup 2+} inhibited biodegradational activity (32 to 97%), and Fe{sup 2+} and Fe{sup 3+} showed a stimulatory effect, but the combination Fe{sup 3+}/Pb{sup 2+} eliminated the Pb{sup 2+} inhibition.

OSTI ID:
490981
Report Number(s):
CONF-950483-; ISBN 1-57477-008-X; TRN: IM9729%%232
Resource Relation:
Conference: 3. international in situ and on-site bioreclamation symposium, San Diego, CA (United States), 24-27 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of Bioremediation of recalcitrant organics; Hinchee, R.E.; Anderson, D.B. [eds.] [Battelle Memorial Inst., Columbus, OH (United States)]; Hoeppel, R.E. [ed.] [Naval Facilities Engineering Services Center, Port Hueneme, CA (United States)]; PB: 378 p.; Bioremediation, Volume 3(7)
Country of Publication:
United States
Language:
English