FISH-mapped CEPH YACs spanning 0 to 46 cM on human chromosome 6
- Yale Univ. School of Medicine, New Haven, CT (United States); and others
Seventy-six CEPH YACs were mapped by fluorescence in situ hybridization (FISH) to human metaphase chromosomes. These clones have been ordered from pter to 46 cM by combining the results of FISH with sequence-tagged site content mapping using data from the public databases. This created a minimal tiling path containing at least 37 Mb of human genomic DNA from 0 to 46 cM on chromosome 6 that contains up to four gaps not greater than 200 kb. These data provide an integration of the FLpter physical map values with cytogenetic band localization and markers on the genetic and radiation hybrid maps. We also assessed YAC chimerism and placed three additional Whitehead contigs within the integrated map. 27 refs., 1 fig., 1 tab.
- OSTI ID:
- 484330
- Journal Information:
- Genomics, Vol. 36, Issue 1; Other Information: PBD: 15 Aug 1996
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
HUMAN CHROMOSOME 6
GENETIC MAPPING
DNA-CLONING
DNA SEQUENCING
CHIMERAS
CHROMOSOMAL ABERRATIONS
DATA BASE MANAGEMENT
HISTOCOMPATIBILITY COMPLEX
GENES
MAN
HEREDITARY DISEASES
DNA HYBRIDIZATION
FLUORESCENCE
BANDING TECHNIQUES
CONTIGS
BIOLOGICAL MARKERS
POLYMERASE CHAIN REACTION