Search for genes having TNT degrading capability for bioremediation. Final report, 25 October 1993-18 September 1996
Several achievements are made through this initial collaborative project These are: (1) creation of enormous capability at this HBcU in enhancing this research; (2) extensive studies on identification of TNT biotransformation products in several bacterial species and one fungal species; (3) identification of several genes which are capable of catalyzing degradation of metabolites of ThT in several microbial species, particularly in the flingus P. chrysosporiwn; (4) isolation and DNA sequence of these genes for probe development; and (5) PCR amplification of 2,4-DNT gene using P. chrysosporiwn genomic DNA as template; (6) DNA:DNA hybridization, (32)P labeled 2,4-DNT dioxygenase gene (used as probe) to anneal with P. chrysosporiwn genomic DNAs; and (7) selection of proper combination of mixed genotypes of bacteria for very efficient degradation of TNT.
- Research Organization:
- Howard Univ., Washington, DC (United States)
- OSTI ID:
- 479102
- Report Number(s):
- AD-A-321239/6/XAB; CNN: Contract DAAH04-93-2-0004; TRN: 71180302
- Resource Relation:
- Other Information: PBD: 22 Nov 1996
- Country of Publication:
- United States
- Language:
- English
Similar Records
Microbial transformations of TNT and DNT in contaminated soils and aquifer materials at Weldon Spring, Missouri
Degradation of Bunker C Fuel Oil by White-Rot Fungi in Sawdust Cultures Suggests Potential Applications in Bioremediation