Integrated physical, genetic, and genic map covering 3 Mb around the human NGF gene (NGFB) at 1p13
- Genetique Moleculaire et Biologie du Development, Villejuif (France); and others
We have characterized 11 overlapping yeast artificial chromosomes (YACs) in the 1p13 region, 8 of them containing the human nerve growth factor (NGF) gene (HGMW-approved symbol NGFB). Sequence-tagged sites (STSs) corresponding to YAC extremities have been designed and used for chromosome assignment on a panel of monochromosomic somatic cell hybrids to check for YAC chimerism, in parallel with analyses by fluorescence in situ hybridization. Determination of end STS content and restriction mapping of the YACs led to the construction of a 3-Mb YAC contig. Four microsatellite markers from the Genethon collection and seven genes known to map to the 1p13 region have been ordered on the contig around the NGF gene. A new gene transcript from the Genexpress catalog has been localized on the contig. This work provides an integrated physical, genetic, and genic map of this chromosome 1 region. It constitutes a basis for determining the structure of the NGF gene and for further characterizing its genetic environment. 36 refs., 2 figs., 4 tabs.
- OSTI ID:
- 478837
- Journal Information:
- Genomics, Vol. 31, Issue 1; Other Information: PBD: 1 Jan 1996
- Country of Publication:
- United States
- Language:
- English
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