UVB-induced mutagenesis in hairless {lambda}lacZ-transgenic mice

Frijhoff, A F.W.; Rebel, H; Mientjes, E J

doi:10.1002/(SICI)1098-2280(1997)29:2<136::AID-EM4>3.0.CO;2-C

Title: UVB-induced mutagenesis in hairless {lambda}lacZ-transgenic mice

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Abstract

UVB-induced mutagenesis was studied in hairless 40.6 transgenic mice (Muta{trademark}Mouse), which contain the {lambda}gt1OlacZ shuttle vector as a target for mutagenesis. Mice were exposed at the dorsal side to either single doses of 200, 500, 800, or 1000 J/m{sup 2} UVB or to two successive irradiations of either 200 and 800 J/m{sup 2} UVB, with intervals of 1,3, or 5 days, or to 800 and 200 J/m{sup 2} UVB with a 5-day interval. At 23 days after the last exposure, lacZ mutant frequencies (MF) were determined in the epidermis. The lacZ MF increased linearly with increasing dose of UVB. The mutagenic effect of two successive irradiations appeared to be additive. The UV-induced mutation spectrum was dominated by G:C{r_arrow}A:T transitions at dipyrimidine sites. DNA-sequence analysis of spontaneously mutated phages showed a diverse spectrum consisting of insertions, deletions and G:C {r_arrow} A:T transitions at CpG sites. the results indicate that the hairless {lambda}lacZ-transgenic mouse is a suitable in vivo model for studying UVB-induced mutations. 29 refs., 5 tabs.

Authors:

Frijhoff, A F.W.; Rebel, H; Mientjes, E J ^[1]

TNO Nutrition and Food Research Institute, Zeist (Netherlands); and others

Publication Date:: Sun Jun 01 00:00:00 EDT 1997

OSTI Identifier:: 478377

Resource Type:: Journal Article

Journal Name:: Environmental and Molecular Mutagenesis

Additional Journal Information:: Journal Volume: 29; Journal Issue: 2; Other Information: PBD: 1997

Country of Publication:: United States

Language:: English

Subject:: 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; MICE; GENETIC RADIATION EFFECTS; MUTATIONS; ULTRAVIOLET RADIATION

Citation Formats


                    Frijhoff, A F.W., Rebel, H, and Mientjes, E J. UVB-induced mutagenesis in hairless {lambda}lacZ-transgenic mice.  United States: N. p., 1997. 
        Web.  doi:10.1002/(SICI)1098-2280(1997)29:2<136::AID-EM4>3.0.CO;2-C.

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                    Frijhoff, A F.W., Rebel, H, & Mientjes, E J. UVB-induced mutagenesis in hairless {lambda}lacZ-transgenic mice.  United States.  https://doi.org/10.1002/(SICI)1098-2280(1997)29:2<136::AID-EM4>3.0.CO;2-C

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                    Frijhoff, A F.W., Rebel, H, and Mientjes, E J. 1997.  
        "UVB-induced mutagenesis in hairless {lambda}lacZ-transgenic mice".  United States.  https://doi.org/10.1002/(SICI)1098-2280(1997)29:2<136::AID-EM4>3.0.CO;2-C.

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@article{osti_478377,

  title        = {UVB-induced mutagenesis in hairless {lambda}lacZ-transgenic mice},

  author       = {Frijhoff, A F.W. and Rebel, H and Mientjes, E J},

  abstractNote = {UVB-induced mutagenesis was studied in hairless 40.6 transgenic mice (Muta{trademark}Mouse), which contain the {lambda}gt1OlacZ shuttle vector as a target for mutagenesis. Mice were exposed at the dorsal side to either single doses of 200, 500, 800, or 1000 J/m{sup 2} UVB or to two successive irradiations of either 200 and 800 J/m{sup 2} UVB, with intervals of 1,3, or 5 days, or to 800 and 200 J/m{sup 2} UVB with a 5-day interval. At 23 days after the last exposure, lacZ mutant frequencies (MF) were determined in the epidermis. The lacZ MF increased linearly with increasing dose of UVB. The mutagenic effect of two successive irradiations appeared to be additive. The UV-induced mutation spectrum was dominated by G:C{r_arrow}A:T transitions at dipyrimidine sites. DNA-sequence analysis of spontaneously mutated phages showed a diverse spectrum consisting of insertions, deletions and G:C {r_arrow} A:T transitions at CpG sites. the results indicate that the hairless {lambda}lacZ-transgenic mouse is a suitable in vivo model for studying UVB-induced mutations. 29 refs., 5 tabs.},

  doi          = {10.1002/(SICI)1098-2280(1997)29:2<136::AID-EM4>3.0.CO;2-C},

  url          = {https://www.osti.gov/biblio/478377},
  journal      = {Environmental and Molecular Mutagenesis},
number       = 2,

  volume       = 29,

  place        = {United States},

  year         = {Sun Jun 01 00:00:00 EDT 1997},

  month        = {Sun Jun 01 00:00:00 EDT 1997}

}

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https://doi.org/10.1002/(SICI)1098-2280(1997)29:2<136::AID-EM4>3.0.CO;2-C

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