Effects of the antibiotic Baytril (enrofloxacin) on PCB-initiated induction of cytochrome P4501A mRNA in sexually mature Atlantic tomcod (Microgadus tomcod)
- Department of Fisheries and Oceans, Moncton, New Brunswick (Canada)
- New York Univ. Medical Center, Tuxedo, NY (United States). Nelson Inst. of Environmental Medicine
The authors used a factorial experimental design to determine whether the antimicrobial Baytril (enrofloxacin) had an effect on induction of cytochrome P4501A (CYP1A) mRNA in Atlantic tomcod. Fish were injected with corn oil, Baytril and corn oil, Baytril and 3,3{prime},4,4{prime}-tetrachlorobiphenyl (PCB) dissolved in corn oil, or just PCB in corn oil. Analysis of gene induction in both sexes considered together showed significant induction by PCB treatment and a significant effect of sex (males higher than females). Because the experiment was carried out just before spawning, their results provide additional evidence for pretranslational suppression of CYP1A induction in spawning females. Separate analysis of the sexes showed that in both males and females, PCB had a significant effect on induction. However, in analyzing the males the authors also observed significant induction by Baytril alone. In addition, the BaytrilPCB combination resulted in the highest induction in any group. These findings demonstrate the potential for confounding results due to exposure of sampled fish to antibiotics, either in a laboratory or field situation.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 474324
- Journal Information:
- Environmental Toxicology and Chemistry, Vol. 16, Issue 2; Other Information: PBD: Feb 1997
- Country of Publication:
- United States
- Language:
- English
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