Bioremediation of MGP soils with mixed fungal and bacterial cultures
- Environmental BioTechnologies, Inc., Menlo Park, CA (United States)
- New England Electric Service, Westborough, MA (United States)
- Electric Power Research Inst., Irving, TX (United States)
This culture selection study examines the degradation of polycyclic automatic hydrocarbon (PAH) by a number of brown- and white-rot fungi and bacterial cultures for the treatment of coal tar wastes. Cultures were screened for naphthalene degradation in shake flasks, and selected organisms were then examined for their ability to degrade a mixture of PAHs in aqueous culture. PAH degradation in the presence of the surfactant, TWEEN 80, was examined for some cultures. Many of the organisms were observed to be resistant to greater than 10 mg/L free cyanide. Solid substrate growth conditions were optimized for the selected fungal cultures in preparation for manufactured gas plant (MGP) soil microcosm experiments. The fungi generally produced more biomass under conditions of acidic to neutral pH, incubation at 30 C with 90% moisture saturation, and with granulated corncobs or alfalfa pellets supplied as a lignocellulosic substrate. Of the cultures screened, nine fungal cultures were selected based on their ability to degrade at least 40% of naphthalene, fluorene, or benzo(a)pyrene in 2 weeks or less. A bacterial culture capable of degrading 30 mg/L of naphthalene in 1 week was also selected, and the cultures were examined further in PAH-degradation studies in contaminated soils.
- Sponsoring Organization:
- Environmental Protection Agency, Washington, DC (United States); Electric Power Research Inst., Palo Alto, CA (United States)
- OSTI ID:
- 474250
- Report Number(s):
- CONF-950483-; ISBN 1-57477-004-7; TRN: 97:008994
- Resource Relation:
- Conference: 3. international in situ and on-site bioreclamation symposium, San Diego, CA (United States), 24-27 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of Bioaugmentation for site remediation; Hinchee, R.E.; Fredrickson, J.; Alleman, B.C. [eds.] [Battelle Memorial Inst., Columbus, OH (United States)]; PB: 272 p.; Bioremediation, Volume 3(3)
- Country of Publication:
- United States
- Language:
- English
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