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Title: Effects of low concentrations of benzene on mouse hematopoietic cells in vivo: A preliminary report

Abstract

Evaluation of benzene-induced hematotoxicity following exposure to low concentrations is important for understanding mechanisms of toxicity and determining the dose response at benzene levels close to the current occupational exposure limit 0 ppm. Male B6C3F1 mice were exposed to 0, 1, 10, 100, or 200 ppm benzene by inhalation for 6 hr/day, 5 days/week for 1, 2, 4, or 8 weeks. At each sampling time, we evaluated primitive and committed progenitor cells, differentiating and maturing lineage-specific cells, and stromal cells in the bone marrow; T and B lymphocytes of the spleen and thymus; micronucleated reticulocytes and erythrocytes; and standard blood parameters. At 100 and 200 ppm benzene, there were rapid and significant reductions in number of reticulocytes in the blood, B lymphocytes in the bone marrow and spleen, and an increased frequency of micronucleated reticulocytes in the bone marrow. At 10 ppm, the only parameter affected was a transient reduction in the number of splenic B lymphocytes. There were no significant effects induced by 1 ppm benzene in this study. The present study suggests numbers of B lymphocytes and maturing erythrocytes, and frequency of micronucleated reticulocytes are sensitive indicators of benzene-induced hematotoxicity and will be useful in further investigation ofmore » the hematotoxicity induced by 10 to 100 ppm benzene. 7 refs., 2 figs.« less

Authors:
; ;  [1]
  1. Chemical Industry Institute of Toxicology, Research Triangle Park, NC (United States)
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
472172
Report Number(s):
CONF-9506288-
Journal ID: EVHPAZ; ISSN 0091-6765; TRN: 97:001626-0024
Resource Type:
Journal Article
Journal Name:
Environmental Health Perspectives
Additional Journal Information:
Journal Volume: 104; Journal Issue: Suppl.6; Conference: Benzene `95: international conference on benzene toxicity, carcinogenesis, and epidemiology, Piscataway, NJ (United States), 17-20 Jun 1995; Other Information: PBD: Dec 1996
Country of Publication:
United States
Language:
English
Subject:
02 PETROLEUM; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; BENZENE; TOXICITY; BONE MARROW; ERYTHROCYTES; INHALATION; LYMPHOCYTES; RETICULOCYTES; OCCUPATIONAL EXPOSURE; EXHAUST GASES; AIR POLLUTION; PETROLEUM REFINERIES; CARCINOGENS; BIOLOGICAL INDICATORS

Citation Formats

Farris, G M, Robinson, S N, and Gaido, K W. Effects of low concentrations of benzene on mouse hematopoietic cells in vivo: A preliminary report. United States: N. p., 1996. Web. doi:10.1289/ehp.961041275.
Farris, G M, Robinson, S N, & Gaido, K W. Effects of low concentrations of benzene on mouse hematopoietic cells in vivo: A preliminary report. United States. https://doi.org/10.1289/ehp.961041275
Farris, G M, Robinson, S N, and Gaido, K W. 1996. "Effects of low concentrations of benzene on mouse hematopoietic cells in vivo: A preliminary report". United States. https://doi.org/10.1289/ehp.961041275.
@article{osti_472172,
title = {Effects of low concentrations of benzene on mouse hematopoietic cells in vivo: A preliminary report},
author = {Farris, G M and Robinson, S N and Gaido, K W},
abstractNote = {Evaluation of benzene-induced hematotoxicity following exposure to low concentrations is important for understanding mechanisms of toxicity and determining the dose response at benzene levels close to the current occupational exposure limit 0 ppm. Male B6C3F1 mice were exposed to 0, 1, 10, 100, or 200 ppm benzene by inhalation for 6 hr/day, 5 days/week for 1, 2, 4, or 8 weeks. At each sampling time, we evaluated primitive and committed progenitor cells, differentiating and maturing lineage-specific cells, and stromal cells in the bone marrow; T and B lymphocytes of the spleen and thymus; micronucleated reticulocytes and erythrocytes; and standard blood parameters. At 100 and 200 ppm benzene, there were rapid and significant reductions in number of reticulocytes in the blood, B lymphocytes in the bone marrow and spleen, and an increased frequency of micronucleated reticulocytes in the bone marrow. At 10 ppm, the only parameter affected was a transient reduction in the number of splenic B lymphocytes. There were no significant effects induced by 1 ppm benzene in this study. The present study suggests numbers of B lymphocytes and maturing erythrocytes, and frequency of micronucleated reticulocytes are sensitive indicators of benzene-induced hematotoxicity and will be useful in further investigation of the hematotoxicity induced by 10 to 100 ppm benzene. 7 refs., 2 figs.},
doi = {10.1289/ehp.961041275},
url = {https://www.osti.gov/biblio/472172}, journal = {Environmental Health Perspectives},
number = Suppl.6,
volume = 104,
place = {United States},
year = {Sun Dec 01 00:00:00 EST 1996},
month = {Sun Dec 01 00:00:00 EST 1996}
}