Identification and analysis of the human murine putative chromatin structure regulator SUPT6H and Supt6h
- Univ. of Michigan Medical Center, Ann Arbor, MI (United States); and others
We have isolated and sequenced SUPT6H and Supt6h, the human and murine homologues of the Saccharomyces cerevisiae and Caenorhabditis elegans genes SPT6 (P using 1603 aa = 6.7 e-{sup 95}) and emb-5 (P using 1603 aa = 7.0 e-{sup 288}), respectively. The human and murine SPT6 homologues are virtually identical, as they share >98% identity and >99% similarity at the protein level. The derived amino acid sequences of these two genes predict a 1603-aa protein (human) and a 1726-bp protein (mouse), respectively. There were several known features, including a highly acidic 5{prime}-region, a degenerate SH2 domain, and a leucine zipper. These features are consistent with a nuclear protein that regulates transcription, whose extreme conservation underscores the likely importance of this gene in mammalian development. Expression of human and murine SPT6 homologues was analyzed by Northern blotting, which revealed a 7.0-kb transcript that was expressed constitutively. The SPT6 homologue was mapped to chromosome 17q11.2 in human by somatic cell hybrid analysis and in situ hybridization. These data indicate that SUPT6H and Supt6h are functionally analogous to SPT6 and emb-5 and may therefore regulate transcription through establishment or maintenance of chromatin structure. 23 refs., 3 figs.
- OSTI ID:
- 466680
- Journal Information:
- Genomics, Vol. 34, Issue 3; Other Information: PBD: 15 Jun 1996
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
GENES
GENE REGULATION
TRANSCRIPTION
BIOLOGICAL EVOLUTION
GENETIC MAPPING
DNA SEQUENCING
DNA-CLONING
GENE MUTATIONS
MAMMALS
ONTOGENESIS
HUMAN CHROMOSOME 17
CHROMATIN
STRUCTURE-ACTIVITY RELATIONSHIPS
SOMATIC CELLS
HYBRIDIZATION
FLUORESCENCE
POLYMERASE CHAIN REACTION
DNA HYBRIDIZATION
SACCHAROMYCES CEREVISIAE
NEMATODES
MICE
AMINO ACID SEQUENCE
HISTONES