Polymorphism and chromosomal localization of the GI-form of human glutathione peroxidase (GPX2) on 14q24.1 by in situ hybridization

Journal Article · Fri Mar 01 00:00:00 EST 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0115· OSTI ID:466602

Chu, Fong-Fong; Esworthy, R S ^[1]; Rohan De Silva, H A ^[2]

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1. City of Hope Medical Center, Duarte, CA (United States)
2. Marie Curie Research Inst., Surrey (United Kingdom); and others

We have isolated a 3.3-kb DNA containing the two exons and a 2.6-kb intron of the human GPX2 gene. This gene encodes the intestinal isoenzyme of glutathione peroxidase, GSHPx-GI. Consistent signals were detected at 14q24.1 when this DNA was used as a probe from fluorescence in situ hybridization on metaphase chromosomes. Based on either single-stranded conformation polymorphism or sequencing analysis, two sites containing DNA polymorphism were found in the intron: the 5{prime} end had a single A/T alteration, and the 3{prime} end had a microsatellite of TC repeats. 11 refs., 3 figs.

• OSTI ID: 466602
• Journal Information: Genomics, Vol. 32, Issue 2; Other Information: PBD: 1 Mar 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
HUMAN CHROMOSOME 14
GENETIC MAPPING
GLUTATHIONE CONJUGATES
GENE MUTATIONS
DNA SEQUENCING
ENZYME ACTIVITY
STRUCTURE-ACTIVITY RELATIONSHIPS
MAN
NERVOUS SYSTEM DISEASES
HEREDITARY DISEASES
EXONS
INTRONS
FLUORESCENCE
NUCLEOTIDES
DNA HYBRIDIZATION
PROBES
ANTIOXIDANTS
POLYMERASE CHAIN REACTION