Microchip electrophoresis for the analysis of biological materials
- Oak Ridge National Lab., TN (United States)
Development of instrumentation for biological and chemical analyses is geared toward extracting more information in a shorter time period at a lower cost. One avenue to achieve these ends is the miniaturization of instrumentation using microfabrication techniques. The primary advantage of such miniaturized devices is the integration of multiple functions required for a complete analysis into a single monolithic unit. Also, by using microfabrication techniques, planar devices with compact geometries enable design of parallel architectures which would handle multiple samples and necessary redundancies. Microfabricated devices that have been demonstrated primarily involve electrically driven separation techniques including capillary electrophoresis, open channel electrochromatography, and capillary gel electrophoresis. Devices that integrate chemical reactions with analysis include capillary electrophoresis with pre- and postseparation derivatization, and restriction digestions of plasmid DNA followed by size analysis of the fragments. The most notable feature of these miniaturized chemical separation devices is the speed with which analyses are performed. These devices have precise fluid control of picoliter volumes simply by proper selection of applied potentials and without the use of mechanical valves. Further motivations for miniaturization and integration of chemical analysis procedures include low void volume connections, reduction of sample and reagent volumes, and rapid diffusional mixing of reagents.
- OSTI ID:
- 466574
- Report Number(s):
- CONF-951017-; TRN: 96:005999-0106
- Resource Relation:
- Conference: 22. annual conference of the Federation of Analytical Chemistry and Spectroscopy Societies, Cincinnati, OH (United States), 15-20 Oct 1995; Other Information: PBD: 1995; Related Information: Is Part Of FACSS XXII - the 22nd annual conference of the Federation of Analytical Chemistry and Spectroscopy Societies; PB: 285 p.
- Country of Publication:
- United States
- Language:
- English
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