Effects of matrix structure/acidity on ion formation in matrix-assisted laser desorption ionization mass spectrometry

Journal Article · Wed Mar 12 00:00:00 EST 1997 · Journal of the American Chemical Society

DOI:https://doi.org/10.1021/ja960269b· OSTI ID:466372

Gimon-Kinsel, M; Preston-Schaffter, L M; Kinsel, G R; Russell, D H ^[1]

---

1. Texas A&M Univ., College Station, TX (United States)

The involvement of ground and excited state proton transfer reactions in matrix-assisted laser desorption ionization (MALDI) of bradykinin and bovine insulin is examined using a series of p-substituted aniline compounds as matrices. Semiempirical calculations of ground and excited state acidity of the p-substituted aniline and anilinium ions are presented. A linear correlation between log (analyte [A + H]{sup +} ion yield) and matrix acidity is obtained. The behavior of the seven p-substituted anilines is discussed in terms of the relationship between matrix compound structure, reactivity, and ability to act as a MALDI matrix. 44 refs., 4 figs., 5 tabs.

• DOE Contract Number: FG05-85ER13434
• OSTI ID: 466372
• Journal Information: Journal of the American Chemical Society, Vol. 119, Issue 10; Other Information: PBD: 12 Mar 1997
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
40 CHEMISTRY
42 ENGINEERING NOT INCLUDED IN OTHER CATEGORIES
INSULIN
MASS SPECTRA
BRADYKININ
ORGANIC COMPOUNDS
PH VALUE
LASER SPECTROSCOPY
DESORPTION
IONIZATION
MICROSTRUCTURE
ANILINE
EXCITED STATES
GROUND STATES